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Product Info Summary
| SKU: | A00325-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Histone deacetylase 2 HDAC2 Antibody
SKU/Catalog Number
A00325-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Histone deacetylase 2 HDAC2 Antibody (Catalog # A00325-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
HDAC2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-Histone deacetylase 2 HDAC2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00325-1)
Host
Rabbit
Contents
HDAC2 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
HDAC2 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human HDAC2. The immunogen is located within the last 50 amino acids of HDAC2.
Cross-reactivity
HDAC2 antibody is human, mouse and rat reactive. At least two isoforms of HDAC2 are known to exist; this antibody will detect both isoforms. HDAC2 antibody is predicted to not cross-react with other members of the HDAC family.
Reactive Species
A00325-1 is reactive to HDAC2 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
55.4 kDa
Background of HDAC2
The histone deacetylase (HDAC) family contains multiple members which are divided into four classes. Class I of the HDAC family comprises four members, HDAC1, 2, 3, and 8, each of which contains a deacetylase domain and exhibits a different, individual substrate specificity and function in vivo (1). HDAC2 was first identified as as a mammalian homolog to the yeast transcriptional regulator RPD3 (2). HDAC2 forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor (2,3). Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events (4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00325-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
HDAC2 antibody can be used for detection of HDAC2 by Western blot at 0.5 - 1 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of HDAC2 in HeLa cell lysate with HDAC2 antibody at (A) 0.5 and (B) 1 μg/ml.
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Immunohistochemistry of HDAC2 in rat testis tissue with HDAC2 antibody at 5 μg/mL.
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Immunofluorescence of HDAC2 in rat testis tissue with HDAC2 antibody at 20 μg/mL.
Specific Publications For Anti-Histone deacetylase 2 HDAC2 Antibody (A00325-1)
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