Anti-HDAC8/Histone Deacetylase 8 Rabbit Monoclonal Antibody

Product Name

Anti-HDAC8/Histone Deacetylase 8 Rabbit Monoclonal Antibody

View all Histone Deacetylase 8/HDAC8 Antibodies

SKU/Catalog Number

M01843-1

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-HDAC8/Histone Deacetylase 8 Rabbit Monoclonal Antibody catalog # M01843-1. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HDAC8/Histone Deacetylase 8 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01843-1)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

HGO-8

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human HDAC8

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01843-1 is reactive to HDAC8 in Human, Mouse, Rat

Applications

M01843-1 is guaranteed for Flow Cytometry, IP, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

50 kDa

Calculated molecular weight

41.758kDa

Background of Histone Deacetylase 8/HDAC8

Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX (K/R) R consensus motif.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:5000-1:20000
ICC/IF 1:50-1:200
IP 1:50
FC 1:50

Validation Images & Assay Conditions

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Western blot analysis of HDAC8 expression in HeLa cell lysate (M01843-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC8 monoclonal antibody (Catalog # M01843-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HDAC8

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IF analysis of HDAC8 using anti-HDAC8 antibody (M01843-1) and anti-Beta Tubulin antibody (M01857-3).
HDAC8 was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated at 1:50 with rabbit anti-HDAC8 Antibody (M01843-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Gene/Protein Information For HDAC8 (Source: Uniprot.org, NCBI)

Alternative Names

EC 3.5.1.98; HD8; HDAC8; HDACL1; Histone Deacetylase 8; histone deacetylase-like 1; RPD3 HDAC8 CDA07, CDLS5, HD8, HDACL1, KDAC8, MRXS6, RPD3, WTS histone deacetylase 8 histone deacetylase 8|histone deacetylase-like 1

For more info on HDAC8, check out the HDAC8 Infographic

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In this infographic, you will see the following information for HDAC8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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