Product Info Summary
SKU: | A06717-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Histone H1 HIST1H1B Antibody
SKU/Catalog Number
A06717-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Histone H1 HIST1H1B Antibody catalog # A06717-1. Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Histone H1 HIST1H1B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06717-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human Histone H1 around the non-phosphorylation site of T17.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06717-1 is reactive to H1-5 in Human, Mouse
Applications
A06717-1 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
22.58kDa
Background of H1-5
Nuclear transcription factor Y subunit beta is a component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blotting validation for Anti-Histone H1 HIST1H1B Antibody A06717-1
Western Blot (WB) analysis of specific cells using Histone H1 polyclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Click image to see more details
Figure 2. Western blotting validation for Anti-Histone H1 HIST1H1B Antibody A06717-1
Western Blot (WB) analysis of 3T3 cells using Histone H1 polyclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For H1-5 (Source: Uniprot.org, NCBI)
Gene Name
H1-5
Full Name
Histone H1.5
Weight
22.58kDa
Superfamily
histone H1/H5 family
Alternative Names
Histone H1.5 H1-5 H1, H1.5, H1B, H1F5, H1s-3, HIST1H1B H1.5 linker histone, cluster member histone H1.5|H1 histone family, member 5|histone 1, H1b|histone H1a|histone H1b|histone H1s-3|histone cluster 1 H1 family member b|histone cluster 1, H1b
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on H1-5, check out the H1-5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for H1-5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Histone H1 HIST1H1B Antibody (A06717-1)
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No publications found for A06717-1
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