Anti-Hsc70 HSPA8 Rabbit Monoclonal Antibody

Product Name

Anti-Hsc70 HSPA8 Rabbit Monoclonal Antibody

View all HSPA8/HSC71/Hsc70 Antibodies

SKU/Catalog Number

M01024

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Hsc70 HSPA8 Rabbit Monoclonal Antibody catalog # M01024. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Hsc70 HSPA8 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01024)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

DBB-8

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Hsc70

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01024 is reactive to HSPA8 in Human, Mouse, Rat

Applications

M01024 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

224-300 kDa

Calculated molecular weight

70.898kDa

Background of HSPA8/HSC71/Hsc70

Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:20
FC 1:30

Validation Images & Assay Conditions

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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.

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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.

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Western blot analysis of Calreticulin expression in (1) HeLa cell lysate; (2) C6 cell lysate.

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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.

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Immunohistochemical analysis of paraffin-embedded Rat ovary, using the Antibody at 1:200 dilution.

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Immunohistochemical analysis of paraffin-embedded Human liver cancer, using the Antibody at 1:500 dilution.

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Immunohistochemical analysis of paraffin-embedded Human breast cancer, using the Antibody at 1:500 dilution.

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Immunohistochemical analysis of paraffin-embedded Rat liver, using the Antibody at 1:200 dilution.

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Immunohistochemical analysis of paraffin-embedded Mouse lung, using the Antibody at 1:200 dilution.

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Immunohistochemical analysis of paraffin-embedded human breast cancer, using Hsc70 Antibody.

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Immunofluorescent analysis using the Antibody at 1:50 dilution.

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Immunofluorescent analysis of MCF7 cells, using Hsc70 Antibody .

Gene/Protein Information For HSPA8 (Source: Uniprot.org, NCBI)

Alternative Names

Heat shock 70 kDa protein 8; heat shock 70kD protein 8; heat shock 70kDa protein 8; heat shock cognate 71 kDa protein; heat shock cognate protein 54; heat shock cognate protein, 71-kDa; HSC54; HSC70; HSC70heat shock 70kd protein 10; HSC71; HSP71; HSP73; HSP73constitutive heat shock protein 70; HSPA10; HSPA8; LAP1; lipopolysaccharide-associated protein 1; LPS-associated protein 1; MGC131511; MGC29929; NIP71; N-myristoyltransferase inhibitor protein 71 HSPA8 HEL-33, HEL-S-72p, HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, LAP-1, LAP1, NIP71 heat shock protein family A (Hsp70) member 8 heat shock cognate 71 kDa protein|LPS-associated protein 1|N-myristoyltransferase inhibitor protein 71|constitutive heat shock protein 70|epididymis luminal protein 33|epididymis secretory sperm binding protein Li 72p|heat shock 70kDa protein 8|heat shock 70kd protein 10|heat shock cognate protein 54|lipopolysaccharide-associated protein 1

For more info on HSPA8, check out the HSPA8 Infographic

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In this infographic, you will see the following information for HSPA8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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