Click image to see more details
Product Info Summary
| SKU: | M00065 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Primate |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, IHC-F, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Hu CD28 Purified Monoclonal Antibody
SKU/Catalog Number
M00065
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD28 Purified Monoclonal Antibody (Catalog# M00065). Tested in Flow Cytometry, IP, WB, IHC-F, ICC application(s). This antibody reacts with Primate, Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD28 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00065)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
CD28.2
Isotype
Mouse IgG1 kappa
Immunogen
DC28.1.3.3 murine T cell hybridoma transfected with human CD28 cDNA. The mouse monoclonal antibody CD28.2 recognizes an extracellular epitope of CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00065 is reactive to CD28 in Human, Primate
Applications
M00065 is guaranteed for Flow Cytometry, IP, IHC-F, ICC, WB Boster Guarantee
Observed Molecular Weight
42 kDa
Calculated molecular weight
25.066kDa
Background of CD28
CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 1-12 µg/ml
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Flow Cytometry validation of CD28 using Anti-Hu CD28 Purified Monoclonal Antibody (M00065).
Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD28 (CD28.2) purified antibody (concentration in sample 1
Click image to see more details
Figure 2. Flow Cytometry validation of CD28 using Anti-Hu CD28 Purified Monoclonal Antibody (M00065).
Separation of human CD28 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD28 (CD28.2) purified antibody (concentration in sample 1Protein Target Info & Infographic
Gene/Protein Information For CD28 (Source: Uniprot.org, NCBI)
Gene Name
CD28
Full Name
T-cell-specific surface glycoprotein CD28
Weight
25.066kDa
Alternative Names
CD28 antigen (Tp44); CD28 antigen; CD28 molecule; CD28; MGC138290; T-cell-specific surface glycoprotein CD28; Tp44 CD28 Tp44 CD28 molecule T-cell-specific surface glycoprotein CD28
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD28, check out the CD28 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD28: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Hu CD28 Purified Monoclonal Antibody (M00065)
Hello CJ!
No publications found for M00065
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Hu CD28 Purified Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-Hu CD28 Purified Monoclonal Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
