Anti-Hu CD314 Purified Azide Free KLRK1 Monoclonal Antibody

Product Name

Anti-Hu CD314 Purified Azide Free KLRK1 Monoclonal Antibody

View all NKG2D/CD314 Antibodies

SKU/Catalog Number

M00661

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Hu CD314 Purified Azide Free KLRK1 Monoclonal Antibody (Catalog# M00661). Tested in Flow Cytometry, IP, IHC-F, Functional Assay application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-Hu CD314 Purified Azide Free KLRK1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00661)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, azide-free

Clonality

Monoclonal

Clone Number

1D11

Isotype

Mouse IgG1 kappa

Immunogen

NKL cell line. The mouse monoclonal antibody 1D11 recognizes an extracellular epitope of CD314 / NKG2D, a 42 kDa C-type lectin-like activating receptor expressed by NK cells, gamma/delta T cells, and CD8+ T cells.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00661 is reactive to KLRK1 in Human

Applications

M00661 is guaranteed for Flow Cytometry, IP, IHC-F, Functional Assay Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

25.274kDa

Background of NKG2D/CD314

CD314, also known as NKG2D (natural killer receptor G2D) or KLRK1 (killer cell lectin-like receptor subfamily K, member 1), is a homodimeric C-type lectin-like activating receptor and costimulator with type II membrane orientation (C teminus extracellular). CD314 homodimers are associated with DAP10, a membrane adaptor protein that signals similar to CD28 by recruitment of phosphatidylinositol 3-kinase. Engagement of CD314 amplifies antigen-specific T cell responses in CD314-positive T cell populations. In NK cells, CD314 is a primary activating receptor. As CD314 ligands the MHC class-I chain-related proteins A and B (MICA, MICB) and UL16-binding proteins (ULBPs) have been identified.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Functional application: Blocking of ligand binding.
Immunohistochemistry: 5-10 μg/ml.
Flow cytometry: 1-4 µg/ml

Validation Images & Assay Conditions

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Figure 1. Flow Cytometry validation of KLRK1 using Anti-Hu CD314 Purified Azide Free KLRK1 Monoclonal Antibody (M00661).

Flow cytometry surface staining pattern of human peripheral blood cells stained using anti-human CD314 (1D11) purified antibody (azide free

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Figure 2. Flow Cytometry validation of KLRK1 using Anti-Hu CD314 Purified Azide Free KLRK1 Monoclonal Antibody (M00661).

Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD314 (1D11) purified antibody (azide free

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Figure 3. Flow Cytometry validation of KLRK1 using Anti-Hu CD314 Purified Azide Free KLRK1 Monoclonal Antibody (M00661).

Separation of human CD314 positive CD56 positive NK cells (red-filled) from CD314 negative CD56 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD314 (1D11) purified antibody (azide free

Gene/Protein Information For KLRK1 (Source: Uniprot.org, NCBI)

Alternative Names

CD314 antigen; CD314; D12S2489E; FLJ17759; FLJ75772; Killer cell lectin-like receptor subfamily K member 1; killer cell lectin-like receptor subfamily K, member 1; KLR; KLRK1; NK cell receptor D; NKG2-D type II integral membrane protein; NKG2D; NKG2-D; NKG2-D-activating NK receptor; NKG2DDNA segment on chromosome 12 (unique) 2489 expressed sequence KLRK1 CD314, D12S2489E, KLR, NKG2-D, NKG2D killer cell lectin like receptor K1 NKG2-D type II integral membrane protein|NK cell receptor D|NKG2-D-activating NK receptor|killer cell lectin-like receptor subfamily K, member 1

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