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Product Info Summary
| SKU: | M01022-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Primate |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, IHC-P, IHC-F, ICC, WB |
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Product info
Product Name
Anti-Hu CD35 Purified CR1 Monoclonal Antibody
SKU/Catalog Number
M01022-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD35 Purified CR1 Monoclonal Antibody (Catalog# M01022-1). Tested in Flow Cytometry, IP, WB, IHC-P, IHC-F, ICC application(s). This antibody reacts with Primate, Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD35 Purified CR1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01022-1)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
E11
Isotype
Mouse IgG1
Immunogen
Acute monocytic leukemia cells and normal blood monocytes. The mouse monoclonal antibody E11 recognizes an extracellular epitope of CD35 (CR1), a type I transmembrane glycoprotein expressed on granulocytes, monocytes, B cells, folicular dendritic cells, erythrocytes, NK and T cell subsets, as well as e.g. on glomerulal podocytes.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01022-1 is reactive to CR1 in Human, Primate
Applications
M01022-1 is guaranteed for Flow Cytometry, IP, IHC-P, IHC-F, ICC, WB Boster Guarantee
Observed Molecular Weight
42 kDa
Calculated molecular weight
223.663kDa
Background of CD35
CD35 (complement receptor 1, CR1) is a monomeric multiple modular cell surface glycoprotein which serves as receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. It is expressed mainly on the surface of granulocytes, monocytes, erythrocytes, B cells and folicular dendritic cells. Besides its role in complement cascade, CD35 is involved in blocking BCR-induced proliferation and the differentiation of B cells to plasmablasts and their Ig production.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (paraffin sections): Heat mediated antigen retrieval.
Immunohistochemistry (frozen sections): Acetone fixation.
Flow cytometry: 1-4 µg/ml
Validation Images & Assay Conditions
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Protein Target Info & Infographic
Gene/Protein Information For CR1 (Source: Uniprot.org, NCBI)
Gene Name
CR1
Full Name
Complement receptor type 1
Weight
223.663kDa
Superfamily
receptors of complement activation (RCA) family
Alternative Names
C3BR; CD35; complement component (3b/4b) receptor 1 (Knops blood group); CR1; KN CR1 C3BR, C4BR, CD35, KN complement C3b/C4b receptor 1 (Knops blood group) complement receptor type 1|C3-binding protein|C3b/C4b receptor|CD35 antigen|Knops blood group antigen|complement component (3b/4b) receptor 1 (Knops blood group)|complement receptor 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CR1, check out the CR1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Hu CD35 Purified CR1 Monoclonal Antibody (M01022-1)
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No publications found for M01022-1
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