Anti-Hu CD47 Purified Monoclonal Antibody

Product Name

Anti-Hu CD47 Purified Monoclonal Antibody

View all CD47 Antibodies

SKU/Catalog Number

M00360-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Hu CD47 Purified Monoclonal Antibody (Catalog# M00360-1). Tested in Flow Cytometry, WB, IHC-F application(s). This antibody reacts with Primate, Human, Pig.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-Hu CD47 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00360-1)

Host

Mouse

Contents

Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

MEM-122

Isotype

Mouse IgM

Immunogen

COS-7 (African green monkey) cells. The antibody MEM-122 reacts with an extracellular epitope of CD47 (Integrin Associated Protein), a 50-55 kDa membrane adhesion molecule (thrombospondin receptor; immunoglobulin supergene family) expressed on leukocytes, platelets and erythrocytes. It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00360-1 is reactive to CD47 in Human, Pig, Primate

Applications

M00360-1 is guaranteed for Flow Cytometry, IHC-F, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

35.214kDa

Background of CD47

CD47 (integrin-associated protein, IAP) is an ubiquitously expressed cell surface transmembrane glycoprotein interacting with several integrins and regulating their functions. Engagement of CD47 by soluble ligands or counter receptors modulates various signaling pathways, such as activation of heterotrimeric G proteins. Binding secreted thrombospondin-1, CD47 counteracts graft vascularization. CD47 acts also as a ligand for CD172a (signal regulatory protein alpha, SIRP alpha), an immune inhibitory receptor on macrophages; this interaction prevents phagocytosis of CD47-positive cells. Moreover, CD47-CD172a system affects cell migration, B cell adhesion and T cell activation. CD47 is also involved in modulation of chondrocyte responses to mechanical signals, and promotes neuronal development, being especially abundant in synapse-rich regions of brain and retina.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow cytometry: 1-4 μg/ml.
Western blotting: Non-reducing conditions.

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Flow Cytometry validation of CD47 using Anti-Hu CD47 Purified Monoclonal Antibody (M00360-1).

Flow cytometry (surface staining) of lysed and washed porcine peripheral blood with purified anti-CD47 (MEM-122) (detection by anti-mouse IgM FITC).
Panel A -porcine PBMC stained with Isotype mouse IgM control (PFR-03; cat. no. 11-803-C100)
Panel B -porcine PBMC sample stained with anti-CD47 (MEM-122)
Cells in the granulocyte gate were used for analysis.

Gene/Protein Information For CD47 (Source: Uniprot.org, NCBI)

Alternative Names

antigen identified by monoclonal 1D8; Antigenic surface determinant protein OA3; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); CD47 antigen; CD47 glycoprotein; CD47 molecule; CD47; IAP; IAPintegrin associated protein; Integrin-associated protein; leukocyte surface antigen CD47; MER6integrin-associated signal transducer; OA3; Protein MER6; Rh-related antigen CD47 IAP, MER6, OA3 CD47 molecule leukocyte surface antigen CD47|CD47 antigen (Rh-related antigen, integrin-associated signal transducer)|CD47 glycoprotein|Rh-related antigen|antigen identified by monoclonal antibody 1D8|antigenic surface determinant protein OA3|integrin associated protein|integrin-associated signal transducer

For more info on CD47, check out the CD47 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CD47: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the CD47 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis