Anti-Hu CD59 Purified Monoclonal Antibody

Product Name

Anti-Hu CD59 Purified Monoclonal Antibody

View all CD59 Antibodies

SKU/Catalog Number

M00914-3

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Hu CD59 Purified Monoclonal Antibody (Catalog# M00914-3). Tested in WB, Flow Cytometry, IP, IHC-P application(s). This antibody reacts with Mouse, Human.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-Hu CD59 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00914-3)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

MEM-43/5

Isotype

Mouse IgG2b

Immunogen

Thymocytes and T lymphocytes. The antibody MEM-43/5 reacts with well defined epitope (around L33) on CD59 (Protectin), a 18-20 kDa glycosylphosphatidylinositol (GPI)-anchored extracellular glycoprotein expressed on the surface of all hematopoietic cells; it is widely present on cells in all tissues. The MEM-43/5 does not compete with most other CD59 antibodies.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00914-3 is reactive to CD59 in Human, Mouse

Applications

M00914-3 is guaranteed for Flow Cytometry, IP, IHC-P, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

14.177kDa

Background of CD59

CD59 (protectin) is a small (18-20 kDa) GPI-anchored ubiquitously expressed inhibitor of the membrane attack complex (MAC). It is thus the key regulator that preserves the autologous cells from terminal effector mechanism of the complement cascade. CD59 associates with C5b-8 complex and thereby counteracts appropriate formation of cytolytic pore within the plasma membrane. CD59 is also an low-affinity ligand of human CD2 and causes T cell costimulation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow cytometry: 0.5-4 μg/ml; positive control: blood.
Immunohistochemistry (paraffin sections): 5 μg/ml.
Western blotting: 1-2 μg/ml; positive control: mouse spleen, non-reducing conditions.

Validation Images & Assay Conditions

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Figure 2. Immunohistochemistry validation of CD59 using Anti-Hu CD59 Purified Monoclonal Antibody (M00914-3).

Immunohistochemistry staining of human skin (paraffin sections) using anti-CD59 (MEM-43/5).
Commercially tested by LifeSpan BioSciences.
For more protocol information of IHC

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Figure 1. Western blotting validation for Anti-Hu CD59 Purified Monoclonal Antibody M00914-3

Western Blotting analysis (non-reducing conditions) of whole cell lysate of HPB-ALL human peripheral blood T cell leukemia cell line using anti-CD59 (MEM-43/5).
Lane 1: original cell lysate
Lane 2: material immunoprecipitated with anti-human CD59 (MEM-43; cat. no. 11-233-C100)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Gene/Protein Information For CD59 (Source: Uniprot.org, NCBI)

Alternative Names

16.3A5; 1F5 antigen; 1F5; 20 kDa homologous restriction factor; CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16; CD59 antigen; CD59 antigen, complement regulatory protein; CD59 glycoprotein; CD59 molecule, complement regulatory protein; CD59; EJ16; EJ30; EJ30, EL32 and G344); EL32; FLJ38134; FLJ92039; G344; HRF20; HRF-20; human leukocyte antigen MIC11; Ly-6-like protein; lymphocytic antigen CD59/MEM43; MACIF; MAC-inhibitory protein; MAC-IP; MEM43 antigen; MEM43; membrane attack complex (MAC) inhibition factor; Membrane attack complex inhibition factor; Membrane in CD59 16.3A5, 1F5, EJ16, EJ30, EL32, G344, HRF-20, HRF20, MAC-IP, MACIF, MEM43, MIC11, MIN1, MIN2, MIN3, MIRL, MSK21, p18-20 CD59 molecule (CD59 blood group) CD59 glycoprotein|1F5 antigen|20 kDa homologous restriction factor|CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16, EJ30, EL32 and G344)|CD59 blood group antigen|CD59 molecule, complement regulatory protein|Ly-6-like protein|MEM43 antigen|T cell-activating protein|human leukocyte antigen MIC11|lymphocytic antigen CD59/MEM43|membrane attack complex (MAC) inhibition factor|membrane attack complex inhibition factor|membrane inhibitor of reactive lysis|protectin|surface anitgen recognized by monoclonal antibody 16.3A5

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