Anti-Hu CD63 Purified Monoclonal Antibody

Product Name

Anti-Hu CD63 Purified Monoclonal Antibody

View all CD63 Antibodies

SKU/Catalog Number

M01080-2

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Hu CD63 Purified Monoclonal Antibody (Catalog# M01080-2). Tested in Flow Cytometry, IP, IHC-P, ICC application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-Hu CD63 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01080-2)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

MEM-259

Isotype

Mouse IgG1

Immunogen

HPB-ALL T cell line. The antibody MEM-259 reacts with an extracellular/luminal epitope of CD63 (LAMP-3), a 40-60 kDa tetraspan glycoprotein expressed by granulocytes, platelets, T cells, monocytes/macrophages and endothelial cells. Cell surface exposition of CD63 is usually activation-dependent.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01080-2 is reactive to CD63 in Human

Applications

M01080-2 is guaranteed for Flow Cytometry, IP, IHC-P, ICC Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

25.637kDa

Background of CD63

CD63 (LAMP-3, lysosome-associated membrane protein-3), a glycoprotein of tetraspanin family, is present in late endosomes, lysosomes and secretory vesicles of various cell types. It is also present in the plasma membrane, usually following cell activation. Hence, it has become an widely used basophil activation marker. In mast cells, however, CD63 exposition does not need their activation. CD63 interacts with integrins and affects phagocytosis and cell migration, it is also involved in H/K-ATPase trafficking regulation of ROMK1 channels. CD63 also serves as a T-cell costimulation molecule. Expression of CD63 can be used for predicting the prognosis in earlier stages of carcinomas.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow cytometry: 2 μg/ml; positive material: activated platelets, neutrophils and basophils.
Immunohistochemistry (paraffin sections): 10 μg/ml; positive tissue: spleen.

Validation Images & Assay Conditions

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Figure 5. Flow Cytometry validation of CD63 using Anti-Hu CD63 Purified Monoclonal Antibody (M01080-2).

Flow cytometry analysis of IgE-activated peripheral blood stained with anti-human CD63 (MEM-259) purified

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Figure 1. Immunocytochemistry staining of CD63 using Anti-Hu CD63 Purified Monoclonal Antibody (M01080-2).

Immunocytochemistry staining of human skin fibroblasts with anti-CD63 (MEM-259; green) after co-incubation of living cells with human Transferrin -Dyomics 547 (cat. no. TFD547; red); cell nuclei stained with DAPI (blue).

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Figure 2. Immunocytochemistry staining of CD63 using Anti-Hu CD63 Purified Monoclonal Antibody (M01080-2).

Immunocytochemistry staining of CD63 in human primary fibroblasts using anti-CD63 (MEM-259; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

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Figure 3. Immunocytochemistry staining of CD63 using Anti-Hu CD63 Purified Monoclonal Antibody (M01080-2).

Immunocytochemistry staining of CD63 in human HeLa cell line using anti-CD63 (MEM-259; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

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Figure 4. Immunohistochemistry validation of CD63 using Anti-Hu CD63 Purified Monoclonal Antibody (M01080-2).

Immunohistochemistry staining of human spleen (paraffin sections) using anti-CD63 (TEM-259).
Commercially tested by LifeSpan BioSciences.
For more protocol information of IHC

Gene/Protein Information For CD63 (Source: Uniprot.org, NCBI)

Alternative Names

CD63 antigen (melanoma 1 antigen); CD63 antigen; CD63 molecule; CD63; Granulophysin; Lamp-3; Lysosomal-associated membrane protein 3; ME491; melanoma 1 antigen; Melanoma-associated antigen ME491; melanoma-associated antigen MLA1; MLA1lysosome-associated membrane glycoprotein 3; Ocular melanoma-associated antigen; OMA81H; tetraspanin-30; Tspan30; tspan-30; TSPAN30granulophysin CD63 LAMP-3, ME491, MLA1, OMA81H, TSPAN30 CD63 molecule CD63 antigen|CD63 antigen (melanoma 1 antigen)|granulophysin|lysosomal-associated membrane protein 3|lysosome-associated membrane glycoprotein 3|melanoma-associated antigen ME491|melanoma-associated antigen MLA1|ocular melanoma-associated antigen|tetraspanin-30|tspan-30

For more info on CD63, check out the CD63 Infographic

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In this infographic, you will see the following information for CD63: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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