Anti-CD63 (Late Endosomes Marker) Monoclonal Antibody

Product Name

Anti-CD63 (Late Endosomes Marker) Monoclonal Antibody

View all CD63 Antibodies

SKU/Catalog Number

M01080

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-CD63 (Late Endosomes Marker) Monoclonal Antibody (Catalog # M01080). Tested in Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human, Mouse.

Storage & Handling

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-CD63 (Late Endosomes Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01080)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: MX-49.129.5

Isotype

IgG1, kappa

Immunogen

Full length CD63 of human origin

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M01080 is reactive to CD63 in Human, Mouse

Applications

M01080 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

25.637kDa

Background of CD63

This monoclonal antibody recognizes protein of 26kDa-60kDa, which is identified as CD63. Its epitope is different from that of MAb LAMP3/529. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (0.5-1ug/ml)
Western Blot (0.5-1ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

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Flow cytometric analysis of NIH/3T3 cells. Black: Cells alone; Green: Isotype Control; Red: PE-labeled Anti-CD63 Mouse Monoclonal Antibody (MX-49.129.5).

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Flow cytometric analysis of MCF-7 cells. Anti-CD63 Mouse Monoclonal Antibody (MX-49.129.5) followed by goat anti-mouse IgG-CF488 (green); unstained cells (gray)

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Flow cytometric analysis of bead-bound exosomes derived from MCF-7 cells. Anti-CD63 Mouse Monoclonal Antibody (MX-49.129.5) followed by goat anti-mouse IgG-CF488 (green); unstained exosomes (gray).

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Flow cytometric analysis of MCF-7 cells. Anti-CD63 Mouse Monoclonal Antibody (MX-49.129.5) followed by goat anti-mouse IgG-CF568 (orange); unstained cells (gray).

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Flow cytometric analysis of bead-bound exosomes derived from MCF-7 cells. Anti-CD63 Mouse Monoclonal Antibody (MX-49.129.5) followed by goat anti-mouse IgG-CF568 (orange); unstained exosomes (gray).

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Immunofluorescence analysis of HeLa cells stained using CF ®488-labeled-CD63 Monoclonal Antibody (MX-49.129.5) (green). F-actin filaments labeled with phalloidin (red), nuclei stained with DAPI (blue).

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Immunofluorescence analysis of PFA-fixed U87MG cells stained using CD63 Mouse Monoclonal Antibody (MX-49.129.5) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).

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Formalin-fixed, paraffin-embedded human melanoma stained with Anti-CD63 Mouse Monoclonal Antibody (MX-49.129.5).

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Formalin-fixed, paraffin-embedded mouse spleen stained with Anti-CD63 Mouse Monoclonal Antibody (MX-49.129.5).

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Formalin-fixed, paraffin-embedded human prostate carcinoma stained with Anti-CD63 Mouse Monoclonal Antibody (MX-49.129.5).

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Western blot analysis of human spleen tissue lysate using Anti-CD63 Mouse Monoclonal Antibody (MX-49.129.5).

Gene/Protein Information For CD63 (Source: Uniprot.org, NCBI)

Alternative Names

CD63 antigen (melanoma 1 antigen); CD63 antigen; CD63 molecule; CD63; Granulophysin; Lamp-3; Lysosomal-associated membrane protein 3; ME491; melanoma 1 antigen; Melanoma-associated antigen ME491; melanoma-associated antigen MLA1; MLA1lysosome-associated membrane glycoprotein 3; Ocular melanoma-associated antigen; OMA81H; tetraspanin-30; Tspan30; tspan-30; TSPAN30granulophysin CD63 LAMP-3, ME491, MLA1, OMA81H, TSPAN30 CD63 molecule CD63 antigen|CD63 antigen (melanoma 1 antigen)|granulophysin|lysosomal-associated membrane protein 3|lysosome-associated membrane glycoprotein 3|melanoma-associated antigen ME491|melanoma-associated antigen MLA1|ocular melanoma-associated antigen|tetraspanin-30|tspan-30

For more info on CD63, check out the CD63 Infographic

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In this infographic, you will see the following information for CD63: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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