Product Info Summary
SKU: | A02693-Dyl550 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | Flow Cytometry |
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Product info
Product Name
Anti-Human ACTN3 DyLight® 550 conjugated Antibody
View all Alpha Actinin 3 Antibodies
SKU/Catalog Number
A02693-Dyl550
Size
100 μg/vial
Form
Liquid
Description
Boster Bio Anti-Human ACTN3 DyLight® 550 conjugated Antibody catalog # A02693-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Cite This Product
Anti-Human ACTN3 DyLight® 550 conjugated Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02693-Dyl550)
Host
Rabbit
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human ACTN3, different from the related mouse sequence by five amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02693-Dyl550 is reactive to ACTN3 in Human
Applications
A02693-Dyl550 is guaranteed for Flow Cytometry Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
103.241kDa
Background of Alpha Actinin 3
Alpha-actinin-3, also known as alpha-actinin skeletal muscle isoform 3 or F-actin cross-linking protein, is a protein that in humans is encoded by the ACTN3 gene. This gene encodes a member of the alpha-actin binding protein gene family. The encoded protein is primarily expressed in skeletal muscle and functions as a structural component of sarcomeric Z line. This protein is involved in crosslinking actin containing thin filaments. An allelic polymorphism in this gene results in both coding and non-coding variants; the reference genome represents the coding allele. The non-functional allele of this gene is associated with elite athlete status.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry, 1-3μg/1x106 cells
Validation Images & Assay Conditions
Click image to see more details
Boster Kit Box
Protein Target Info & Infographic
Gene/Protein Information For ACTN3 (Source: Uniprot.org, NCBI)
Gene Name
ACTN3
Full Name
Alpha-actinin-3
Weight
103.241kDa
Superfamily
alpha-actinin family
Alternative Names
actinin, alpha 3; Alpha-actinin skeletal muscle isoform 3; alpha-actinin skeletal muscle; alpha-actinin-3; F-actin cross-linking protein; MGC117002; MGC117005 ACTN3 ACTN3D actinin alpha 3 alpha-actinin-3|F-actin cross-linking protein|alpha-actinin skeletal muscle
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ACTN3, check out the ACTN3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ACTN3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human ACTN3 DyLight® 550 conjugated Antibody (A02693-Dyl550)
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-Human ACTN3 DyLight® 550 conjugated Antibody
Question
Is a blocking peptide available for product anti-Human ACTN3 DyLight® 550 conjugated antibody (A02693-Dyl550)?
W. Roberts
Verified customer
Asked: 2020-01-16
Answer
We do provide the blocking peptide for product anti-Human ACTN3 DyLight® 550 conjugated antibody (A02693-Dyl550). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-16
Question
Here is the WB image, lot number and protocol we used for skeletal muscle tissue of rectus abdominis using anti-Human ACTN3 DyLight® 550 conjugated antibody A02693-Dyl550. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-08
Question
Do you have a BSA free version of anti-Human ACTN3 DyLight® 550 conjugated antibody A02693-Dyl550 available?
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Human ACTN3 DyLight® 550 conjugated antibody A02693-Dyl550 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-08-09
Question
Can you help my question with product A02693-Dyl550, anti-Human ACTN3 DyLight® 550 conjugated antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
G. Roberts
Verified customer
Asked: 2016-03-10
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02693-Dyl550 anti-Human ACTN3 DyLight® 550 conjugated antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-03-10