Anti-Human ACTN3 DyLight® 550 conjugated Antibody

Alpha Actinin 3 antibody

Boster Bio Anti-Human ACTN3 DyLight® 550 conjugated Antibody catalog # A02693-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.

Product Info Summary

SKU: A02693-Dyl550
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry

Product Name

Anti-Human ACTN3 DyLight® 550 conjugated Antibody

View all Alpha Actinin 3 Antibodies

SKU/Catalog Number

A02693-Dyl550

Size

100 μg/vial

Form

Liquid

Description

Boster Bio Anti-Human ACTN3 DyLight® 550 conjugated Antibody catalog # A02693-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Cite This Product

Anti-Human ACTN3 DyLight® 550 conjugated Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02693-Dyl550)

Host

Rabbit

Contents

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human ACTN3, different from the related mouse sequence by five amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A02693-Dyl550 is reactive to ACTN3 in Human

Applications

A02693-Dyl550 is guaranteed for Flow Cytometry Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

103.241kDa

Background of Alpha Actinin 3

Alpha-actinin-3, also known as alpha-actinin skeletal muscle isoform 3 or F-actin cross-linking protein, is a protein that in humans is encoded by the ACTN3 gene. This gene encodes a member of the alpha-actin binding protein gene family. The encoded protein is primarily expressed in skeletal muscle and functions as a structural component of sarcomeric Z line. This protein is involved in crosslinking actin containing thin filaments. An allelic polymorphism in this gene results in both coding and non-coding variants; the reference genome represents the coding allele. The non-functional allele of this gene is associated with elite athlete status.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry, 1-3μg/1x106 cells

Validation Images & Assay Conditions

Gene/Protein Information For ACTN3 (Source: Uniprot.org, NCBI)

Gene Name

ACTN3

Full Name

Alpha-actinin-3

Weight

103.241kDa

Superfamily

alpha-actinin family

Alternative Names

actinin, alpha 3; Alpha-actinin skeletal muscle isoform 3; alpha-actinin skeletal muscle; alpha-actinin-3; F-actin cross-linking protein; MGC117002; MGC117005 ACTN3 ACTN3D actinin alpha 3 alpha-actinin-3|F-actin cross-linking protein|alpha-actinin skeletal muscle

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ACTN3, check out the ACTN3 Infographic

ACTN3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ACTN3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-Human ACTN3 DyLight® 550 conjugated Antibody

Question

Is a blocking peptide available for product anti-Human ACTN3 DyLight® 550 conjugated antibody (A02693-Dyl550)?

W. Roberts

Verified customer

Asked: 2020-01-16

Answer

We do provide the blocking peptide for product anti-Human ACTN3 DyLight® 550 conjugated antibody (A02693-Dyl550). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-01-16

Question

Here is the WB image, lot number and protocol we used for skeletal muscle tissue of rectus abdominis using anti-Human ACTN3 DyLight® 550 conjugated antibody A02693-Dyl550. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-01-08

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-01-08

Question

Do you have a BSA free version of anti-Human ACTN3 DyLight® 550 conjugated antibody A02693-Dyl550 available?

Verified Customer

Verified customer

Asked: 2019-08-09

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Human ACTN3 DyLight® 550 conjugated antibody A02693-Dyl550 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-08-09

Question

Can you help my question with product A02693-Dyl550, anti-Human ACTN3 DyLight® 550 conjugated antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

G. Roberts

Verified customer

Asked: 2016-03-10

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02693-Dyl550 anti-Human ACTN3 DyLight® 550 conjugated antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2016-03-10

Size

Total: $515

SKU:A02693-Dyl550

Backordered.

Lead time for this item is typically 2-3 weeks

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A02693-Dyl550
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$515.00

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