Introduction
Clone APN1464 recognizes cell surface CD13 antigen, a 150kDa membrane glycoprotein. The CD13 antigen is
highly expressed mostly on myeloid-derived hematopoietic cells including granulocytes, monocytes, mast cells,
and GM-progenitor cells. CD13 abundantly expresses on most of the malignant cells of myeloid origin such as
AML, CML and also on smaller subset of cancer cells of lymphoid origin. Normal lymphocytes, platelets and
erythrocytes do not express CD13. CD13 plays important role in metabolism of biologically active peptides, in
phagocytosis, and in bactericidal/tumoricidal immune process. It also serves as a receptor for human
coronaviruses (HCV).
This antibody is routinely tested by flow cytometric analysis. Flow cytometry and other applications were tested during antibody development by CapricoBio or are reported in the literature.
Application Information
Each lot of this antibody has been pre-titrated and tested by flow cytometric analysis using human PBMCs. 5μl of
this product is found sufficient for staining 1 million cells in a 100μl staining volume or 100μl of whole blood.
However, it is recommended that antibody reactivity be empirically titrated for optimal performance in the
application of interest.
Overview
| Product Name |
Anti-human CD13 ANPEP Monoclonal Antibody PerCP-Cy5.5 Conjugated, Flow Validated
See more Aminopeptidase N/CD13 products |
| Catalog# |
FC02591-PerCP-Cy5.5 |
| Pack Size |
100 Tests, 200 Tests |
| Storage & Handling |
4°C and protect from prolonged exposure to light. Do not freeze. |
| Description |
Boster Bio Anti-human CD13 ANPEP Monoclonal Antibody PerCP-Cy5.5 Conjugated, Flow Validated catalog # FC02591-PerCP-Cy5.5. Tested in Flow Cytometry applications. This antibody reacts with Human. Supplied as 100 Tests, 200 Tests in Liquid form antibody. |
| Cite This Product |
Anti-human CD13 ANPEP Monoclonal Antibody PerCP-Cy5.5 Conjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC02591-PerCP-Cy5.5)
|
| Antibodies Validation |
Antibodies Validation Information
|
Product Specs
| Host |
Mouse |
| Reactive Species |
Human |
| Applications |
Flow Cytometry
*Our Boster Guarantee covers the use of this product in the above
tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products. |
| Immunogen |
Recombinant human CD13 protein |
Gene/Protein Basic Information For ANPEP (Source: Uniprot.org, NCBI)
| Uniprot Id | P15144 |
|---|
| NCBI Gene Id | 290 |
|---|
| Species Of This Entry | Human |
|---|
| Gene Name | ANPEP |
|---|
| Protein Name | Aminopeptidase N |
|---|
| Superfamily | peptidase M1 family |
|---|
| Alternative Names | Aminopeptidase N/CD13|alanyl (membrane) aminopeptidase; Alanyl aminopeptidase; Aminopeptidase M; Aminopeptidase N; ANPEP; AP-M; APN; AP-N; CD13 antigen; CD13; CD13APN; EC 3.4.11; EC 3.4.11.2; gp150; LAP1; Microsomal aminopeptidase; Myeloid plasma membrane glycoprotein CD13; p150; PEPN; PEPNhAPN |
|---|
| Molecular Weight | 109540 |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on ANPEP, check out the ANPEP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for ANPEP: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the ANPEP infographic
Our Boster Quality Guarantee for Anti-human CD13 ANPEP Monoclonal Antibody PerCP-Cy5.5 Conjugated, Flow Validated covers its use in the following applications.
User need to optimize the dilution ratio for this antibody
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Lymphocytes gated PBMCs stained with
FITC conjugated anti-human CD7 (clone
4H9, red histogram). Lymphocytes gated
PBMCs stained with IgG2a FITC isotype
control (blue histogram).
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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