Anti-human CD5 Monoclonal Antibody PerCP-Cy5.5 Conjugated, Flow Validated

CD5 antibody

Boster Bio Anti-human CD5 Monoclonal Antibody PerCP-Cy5.5 Conjugated, Flow Validated (Catalog# FC00480-PerCP-Cy5.5). Tested in Flow Cytometry application(s). This antibody reacts with Human.

Product Info Summary

SKU: FC00480-PerCP-Cy5.5
Size: 100 tests, 200 tests
Reactive Species: Human
Host: Mouse
Application: Flow Cytometry

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Product Name

Anti-human CD5 Monoclonal Antibody PerCP-Cy5.5 Conjugated, Flow Validated

View all CD5 Antibodies

SKU/Catalog Number

FC00480-PerCP-Cy5.5

Size

100 tests, 200 tests

Form

Liquid

Description

Boster Bio Anti-human CD5 Monoclonal Antibody PerCP-Cy5.5 Conjugated, Flow Validated (Catalog# FC00480-PerCP-Cy5.5). Tested in Flow Cytometry application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-human CD5 Monoclonal Antibody PerCP-Cy5.5 Conjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC00480-PerCP-Cy5.5)

Host

Mouse

Contents

PBS pH 7.2, 0.2% (w/v) BSA, 0.09% (w/v) sodium azide

Clonality

Monoclonal

Clone Number

Clone: OKT1

Isotype

IgG1,k

Immunogen

Human peripheral blood lymphocytes

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

FC00480-PerCP-Cy5.5 is reactive to CD5 in Human

Applications

FC00480-PerCP-Cy5.5 is guaranteed for Flow Cytometry Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

54.578kDa

Background of CD5

CD5, also known as T1 or LEU1, is a cluster of differentiation found on a subset of IgM-secreting B cells called B-1 cells, and also on T cells. It is mapped to 11q12.2. Although CD5 can be found on both T cells and B cells, T cells express higher levels of CD5 than B cells. CD5 was used as a T-cell marker until monoclonal antibodies against CD3 were developed. CD5 is upregulated on T cells upon strong activation. In the thymus, there is a correlation with CD5 expression and strength of the interaction of the T cell towards self-peptides. Nevertheless, CD5 serves to mitigate activating signals from the BCR so that the B-1 cells can only be activated by very strong stimuli (such as bacterial proteins) and not by normal tissue proteins.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

User needs to optimize the dilution ratio for this antibody.

Validation Images & Assay Conditions

Gene/Protein Information For CD5 (Source: Uniprot.org, NCBI)

Gene Name

CD5

Full Name

T-cell surface glycoprotein CD5

Weight

54.578kDa

Alternative Names

CD5 antigen (p56-62); CD5 antigen; CD5 molecule; CD5; LEU1T-cell surface glycoprotein CD5; Lymphocyte antigen T1/Leu-1; T1 CD5 LEU1, T1 CD5 molecule T-cell surface glycoprotein CD5|CD5 antigen (p56-62)|epididymis secretory sperm binding protein|lymphocyte antigen T1/Leu-1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CD5, check out the CD5 Infographic

CD5 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CD5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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10 Customer Q&As for Anti-human CD5 Monoclonal Antibody PerCP-Cy5.5 Conjugated, Flow Validated

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukocyte using anti-human CD5 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC00480-PerCP-Cy5.5. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-04-29

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-04-29

Question

Would FC00480-PerCP-Cy5.5 anti-human CD5 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-04-14

Answer

It shows on the product datasheet, FC00480-PerCP-Cy5.5 anti-human CD5 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated as been tested on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-04-14

Question

I have attached the WB image, lot number and protocol we used for leukocyte using anti-human CD5 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC00480-PerCP-Cy5.5. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-10-29

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-10-29

Question

I see that the anti-human CD5 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC00480-PerCP-Cy5.5 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-08-16

Answer

You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-08-16

Question

Can you help my question with product FC00480-PerCP-Cy5.5, anti-human CD5 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-07-24

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free FC00480-PerCP-Cy5.5 anti-human CD5 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-07-24

Question

Is a blocking peptide available for product anti-human CD5 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated (FC00480-PerCP-Cy5.5)?

Verified Customer

Verified customer

Asked: 2019-03-29

Answer

We do provide the blocking peptide for product anti-human CD5 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated (FC00480-PerCP-Cy5.5). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-03-29

Question

I was wanting to use your anti-human CD5 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated for Flow Cytometry for human leukocyte on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human leukocyte identification?

Verified Customer

Verified customer

Asked: 2019-03-11

Answer

You can see on the product datasheet, FC00480-PerCP-Cy5.5 anti-human CD5 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated has been validated for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human leukocyte in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-03-11

Question

Does anti-human CD5 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC00480-PerCP-Cy5.5 work for Flow Cytometry with leukocyte?

Verified Customer

Verified customer

Asked: 2017-10-19

Answer

According to the expression profile of leukocyte, CD5 is highly expressed in leukocyte. So, it is likely that anti-human CD5 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC00480-PerCP-Cy5.5 will work for Flow Cytometry with leukocyte.

Boster Scientific Support

Answered: 2017-10-19

Question

Is there a BSA free version of anti-human CD5 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC00480-PerCP-Cy5.5 available?

M. Parker

Verified customer

Asked: 2014-08-04

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-human CD5 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC00480-PerCP-Cy5.5 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2014-08-04

Question

I am interested in to test anti-human CD5 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC00480-PerCP-Cy5.5 on human leukocyte for research purposes, then I may be interested in using anti-human CD5 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC00480-PerCP-Cy5.5 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

L. Walker

Verified customer

Asked: 2014-01-14

Answer

The products we sell, including anti-human CD5 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC00480-PerCP-Cy5.5, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2014-01-14

Order DetailsPrice
FC00480-PerCP-Cy5.5-100tests

100 tests

$360
FC00480-PerCP-Cy5.5-200tests

200 tests

$612

Free Secondary Antibody

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FC00480-PerCP-Cy5.5
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$360.00

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