Anti-Human IRS1 DyLight® 488 conjugated Antibody(monoclonal, 10I3)

Product Name

Anti-Human IRS1 DyLight® 488 conjugated Antibody(monoclonal, 10I3)

View all IRS1 Antibodies

SKU/Catalog Number

M00268-Dyl488

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Human IRS1 DyLight® 488 conjugated Antibody (monoclonal, 10I3) catalog # M00268-Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Cite This Product

Anti-Human IRS1 DyLight® 488 conjugated Antibody(monoclonal, 10I3) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00268-Dyl488)

Host

Mouse

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Monoclonal

Clone Number

10I3

Isotype

Mouse IgG2a

Immunogen

E.coli-derived human IRS1 recombinant protein (Position: S1041-Q1242). Human IRS1 shares 78% and 80% amino acid (aa) sequence identity with mouse and rat IRS1, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

M00268-Dyl488 is reactive to IRS1 in Human

Applications

M00268-Dyl488 is guaranteed for Flow Cytometry Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

131.591kDa

Background of IRS1

Insulin receptor substrate 1 (IRS-1) is a signalling adapter protein that in humans is encoded by the IRS-1 gene. It is mapped to 2q36.3. This gene exhibited no intrinsic enzyme activity, and it can serve as a docking protein involved in binding and activating other signal transduction molecules after being phosphorylated on tyrosine by insulin receptor kinase. IRS1 plays a key role in transmitting signals from the insulin and insulin-like growth factor-1 (IGF-1) receptors to intracellular pathways PI3K/Akt and Erk MAP kinase pathways. IRS1 also has important biological function for both metabolic and mitogenic (growth promoting) pathways. In addition to those, IRS1 is a key regulator of PI3K within malignant cells.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry, 1-3μg/1x10⁶ cells, Human

Validation Images & Assay Conditions

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Figure 1. Flow Cytometry analysis of A549 cells using anti-Human IRS1 antibody (M04544-Dyl488).
Overlay histogram showing A549 cells stained with M04544-Dyl488 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Human IRS1 Antibody (M04544-Dyl488,1μg/1x10⁶ cells) for 30 min at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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Figure 2. Flow Cytometry analysis of SiHa cells using anti-Human IRS1 antibody (M00480-Dyl488).
Overlay histogram showing SiHa cells stained with M00480-Dyl488 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Human IRS1 Antibody (M00480-Dyl488,1μg/1x10⁶ cells) for 30 min at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For IRS1 (Source: Uniprot.org, NCBI)

Alternative Names

HIRS-1; insulin receptor substrate 1; IRS1; IRS-1 IRS1 HIRS-1 insulin receptor substrate 1 insulin receptor substrate 1

For more info on IRS1, check out the IRS1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for IRS1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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