Product Info Summary
SKU: | M01389-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human |
Host: | Mouse |
Application: | ELISA, Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-HXK II HK2 Monoclonal Antibody
View all Hexokinase 2 Antibodies
SKU/Catalog Number
M01389-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-HXK II HK2 Monoclonal Antibody catalog # M01389-1. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HXK II HK2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01389-1)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
3D3
Isotype
IgG
Immunogen
Purified recombinant fragment of human HXK II expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
M01389-1 is reactive to HK2 in Human
Applications
M01389-1 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
102.38kDa
Background of Hexokinase 2
TRPC6, also known as TRP6, short transient receptor potential channel 6 and transient receptor potential cation channel subfamily C member 6, is thought to form a receptor-activated non-selective calcium permeant cation channel. TRPC6 is probably operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. It is activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C and may not to be activated by intracellular calcium store depletion. Defects in this gene are a cause of focal segmental glomerulosclerosis (FSGS). Expression of this protein has been reported in tissues such as placenta, lung, spleen, ovary, small intestine, and renal podocytes. Immunohistochemistry studies using polyclonal antibodies to this target have shown moderate to strong staining in cell types such as neurons, breast, respiratory, squamous and prostate epithelium, epidermis, placental trophoblasts, dendritic cells, and subsets of immune cells, and faint to moderate staining of adrenal, colon, ganglion cells, hepatocytes, heart, and testis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:200-1:1000
FC 1:200-1:400
ELISA 1:10000
Validation Images & Assay Conditions
Click image to see more details
Figure 3. Flow Cytometry validation of HK2 using Anti-HXK II HK2 Monoclonal Antibody (M01389-1).
Flow cytometric (FCM) analysis of K562 cells using HXK II Monoclonal Antibody (green) and negative control (purple).Click image to see more details
Figure 2. Immunohistochemistry validation of HK2 using Anti-HXK II HK2 Monoclonal Antibody (M01389-1).
Immunohistochemistry (IHC) analysis of paraffin-embedded esophagus cancer tissues (left) and Human Lung Cancer (right) with DAB staining using HXK II Monoclonal Antibody.For more protocol information of IHC
Click image to see more details
Figure 1. Western blotting validation for Anti-HXK II HK2 Monoclonal Antibody M01389-1
Western Blot (WB) analysis using HXK II Monoclonal Antibody against Jurkat (1)Protein Target Info & Infographic
Gene/Protein Information For HK2 (Source: Uniprot.org, NCBI)
Gene Name
HK2
Full Name
Hexokinase-2
Weight
102.38kDa
Superfamily
hexokinase family
Alternative Names
DKFZp686M1669; EC 2.7.1; Hexokinase 2; Hexokinase type II; HK2; HKII; HXK2; Muscle form hexokinase; muscle HK2 HKII, HXK2 hexokinase 2 hexokinase-2|hexokinase type II|hexokinase-2, muscle|hexokinase-B|muscle form hexokinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HK2, check out the HK2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HXK II HK2 Monoclonal Antibody (M01389-1)
Hello CJ!
M01389-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Chen J,Wu D,Dong Z,Chen A,Liu S.The expression and role of glycolysis-associated molecules in infantile hemangioma.Life Sci.2020 Oct 15;259:118215.doi:10.1016/j.lfs.2020.118215.Epub 2020 Aug 5.PMID:32768579.
Species: Human
M01389-1 usage in article: APP:IHC, SAMPLE:NORMAL AND TUMOR SKIN TISSUE, DILUTION:1:200
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Customer Reviews
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