Product Info Summary
SKU: | M04784 |
---|---|
Size: | 100ul |
Reactive Species: | Human |
Host: | Mouse |
Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-Hyaluronan synthase 1 HAS1 Monoclonal Antibody
View all Hyaluronan synthase 1 Antibodies
SKU/Catalog Number
M04784
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Hyaluronan synthase 1 HAS1 Monoclonal Antibody catalog # M04784. Tested in ELISA, IF, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Hyaluronan synthase 1 HAS1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04784)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
3E10
Isotype
IgG
Immunogen
Purified recombinant fragment of human HAS1 expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
M04784 is reactive to HAS1 in Human
Applications
M04784 is guaranteed for ELISA, IF, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
64.832kDa
Background of Hyaluronan synthase 1
Anti Mesothelin Antibody recognizes Mesothelin that is a glycosyl-phosphatidylinositol–anchored glycoprotein present on the cell surface of various human solid tumors. The mesothelin (MSLN) gene encodes a 71-kDa precursor protein that is processed to a 40-kDa glycosylphosphatidylinositol–anchored protein that composes the mature portion and an NH2 terminal 31-kDa fragment called megakaryocyte-potentiating factor that is released from the cell. Mesothelin is a tumor differentiation antigen present at low levels on a restricted set of normal adult tissues, such as mesothelium, but aberrantly over expressed in mesotheliomas, ovarian, and pancreatic cancers. The biological functions of mesothelin remain elusive. A recent study showed that mesothelin binds to MUC16/CA125, and that this interaction mediates cell adhesion, suggesting that there may be an important role for MUC16/CA125 and mesothelin in the metastatic spread of ovarian cancer.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IF 1:200-1:1000
ELISA 1:10000
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunofluorescent staining data of HAS1 using Anti-Hyaluronan synthase 1 HAS1 Monoclonal Antibody (M04784).
Immunofluorescence (IF) analysis of U251 cells using HAS1 Monoclonal Antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.Click image to see more details
Figure 1. Western blotting validation for Anti-Hyaluronan synthase 1 HAS1 Monoclonal Antibody M04784
Western Blot (WB) analysis using HAS1 Monoclonal Antibody against recombinant protein of human HAS1 (aa70-243).Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For HAS1 (Source: Uniprot.org, NCBI)
Gene Name
HAS1
Full Name
Hyaluronan synthase 1
Weight
64.832kDa
Superfamily
NodC/HAS family
Alternative Names
HA synthase 1; HAS; huHAS1; hyaluronan synthase 1; hyaluronate synthase 1; hyaluronic acid synthase 1 HAS1 HAS hyaluronan synthase 1 hyaluronan synthase 1|HA synthase 1|hyaluronate synthase 1|hyaluronic acid synthase 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HAS1, check out the HAS1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HAS1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Hyaluronan synthase 1 HAS1 Monoclonal Antibody (M04784)
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No publications found for M04784
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