Anti-ICAM2 Antibody Picoband™

Product Name

Anti-ICAM2 Antibody Picoband™

View all ICAM-2/CD102 Antibodies

SKU/Catalog Number

A03965

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-ICAM2 Antibody Picoband™ catalog # A03965. Tested in ELISA, WB applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ICAM2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03965)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human ICAM2 recombinant protein (Position: K25-Q223).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A03965 is reactive to ICAM2 in Human, Rat

Applications

A03965 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

55 kDa

Calculated molecular weight

30.654kDa

Background of ICAM-2/CD102

Intercellular adhesion molecule 2 (ICAM2), also known as CD102 (Cluster of Differentiation 102), is a human gene. The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance. Several transcript variants encoding the same protein have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of ICAM2 using anti-ICAM2 antibody (A03965).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysate,
Lane 2: rat thymus tissue lysate,
Lane 3: rat lung tissue lysate,
Lane 4: rat heart tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ICAM2 antigen affinity purified polyclonal antibody (Catalog # A03965) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ICAM2 at approximately 55KD. The expected band size for ICAM2 is at 31KD.

Gene/Protein Information For ICAM2 (Source: Uniprot.org, NCBI)

Alternative Names

CD102 antigen; CD102; ICAM2; ICAM-2; intercellular adhesion molecule 2 ICAM2 CD102 intercellular adhesion molecule 2 intercellular adhesion molecule 2|ICAM-2

For more info on ICAM2, check out the ICAM2 Infographic

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In this infographic, you will see the following information for ICAM2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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