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Product Info Summary
| SKU: | A31846-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-ICT1 Antibody (C-term)
SKU/Catalog Number
A31846-1
Size
80 µl
Description
Boster Bio Anti-ICT1 Antibody (C-term) (Catalog # A31846-1). Tested in IHC-P, WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-ICT1 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A31846-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This ICT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 153-179 amino acids from the C-terminal region of human ICT1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A31846-1 is reactive to MRPL58 in Human
Applications
A31846-1 is guaranteed for IHC-P, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
23.63kDa
Background of ICT
Essential peptidyl-tRNA hydrolase component of the mitochondrial large ribosomal subunit. Acts as a codon-independent translation release factor that has lost all stop codon specificity and directs the termination of translation in mitochondrion, possibly in case of abortive elongation. May be involved in the hydrolysis of peptidyl-tRNAs that have been prematurely terminated and thus in the recycling of stalled mitochondrial ribosomes.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:25
Validation Images & Assay Conditions
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ICT1 Antibody (C-term) western blot analysis in Hela,Jurkat,U251,MDA-MB453,NCI-H292,K562,ZR-75-1 cell line lysates (35ug/lane).This demonstrates the ICT1 antibody detected the ICT1 protein (arrow).
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Immunohistochemical analysis of paraffin-embedded H. colorectal carcinoma section using ICT1 Antibody (C-term). A31846-1 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
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Immunohistochemical analysis of paraffin-embedded H. skin section using ICT1 Antibody (C-term). A31846-1 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Protein Target Info & Infographic
Gene/Protein Information For MRPL58 (Source: Uniprot.org, NCBI)
Gene Name
MRPL58
Full Name
Peptidyl-tRNA hydrolase ICT1, mitochondrial
Weight
23.63kDa
Superfamily
prokaryotic/mitochondrial release factor family
Alternative Names
Digestion substraction 1; DS-1DS1; EC 3.1.1.29; Immature colon carcinoma transcript 1 protein; immature colon carcinoma transcript 1; peptidyl-tRNA hydrolase ICT1, mitochondrial MRPL58 DS-1, DS1, ICT1, MRP-L58 mitochondrial ribosomal protein L58 peptidyl-tRNA hydrolase ICT1, mitochondrial|39S ribosomal protein L58, mitochondrial|digestion substraction 1|immature colon carcinoma transcript 1 protein|mitochondrial large ribosomal subunit protein ICT1|mitochondrial large ribosomal subunit protein mL62
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MRPL58, check out the MRPL58 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MRPL58: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ICT1 Antibody (C-term) (A31846-1)
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