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Product Info Summary
| SKU: | A00510 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-IDH2 Antibody
SKU/Catalog Number
A00510
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-IDH2 Antibody (Catalog # A00510). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-IDH2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00510)
Host
Rabbit
Contents
IDH2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
IDH2 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human IDH2. The immunogen is located within the first 50 amino acids of IDH2.
Reactive Species
A00510 is reactive to IDH2 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
50.9 kDa
Background of IDH2
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Two NADP(+)-dependent isocitrate dehydrogenases have been found as homodimer: IDH1 is predominantly cytosolic and peroxisomal and IDH2 is mitochondrial. Both IDH1 and IDH2 play significant roles in the tricarboxylic acid cycle (TCA), and defects in IDH1 as well as IDH2 have been implicated in the development of glioma as well as other malignancies.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00510 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IDH2 antibody can be used for detection of IDH2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of IDH2 in human heart tissue lysate with IDH2 antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of IDH2 in mouse heart tissue with IDH2 antibody at 5 μg/mL.
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Immunofluorescence of IDH2 in mouse heart tissue with IDH2 antibody at 20 μg/mL.
Specific Publications For Anti-IDH2 Antibody (A00510)
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