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Product Info Summary
| SKU: | A04014 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-IEX-1 IER3 Antibody
SKU/Catalog Number
A04014
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-IEX-1 IER3 Antibody (Catalog # A04014). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
IEX-1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-IEX-1 IER3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04014)
Host
Rabbit
Contents
IEX-1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
IEX1 antibody was raised against a 24 amino acid synthetic peptide near the center of human IEX1. The immunogen is located within amino acids 40 - 90 of IEX-1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
ADRM1 antibody is human, mouse and rat reactive. At least two isoforms of ADRM1 are know to exist; this antibody will detect both isoforms.
Reactive Species
A04014 is reactive to IER3 in Human, Mouse
Applications
A04014 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
16.903kDa
Background of IER3
IEX-1 is a stress inducible gene that is induced by ionizing radiation, ultraviolet radiation, and a variety of growth factors, i.e., FAS and TNF-α. IEX-1 is widely expressed in epithelial and endocrine tissues, as well as in vascular endothelium. It plays an important role in the regulation of cellular growth, cell death and oncogenesis. IEX-1 is precisely regulated by multiple transcription factors such as p53, NF-κB/rel, Sp1 and c-Myc, to ensure rapid and transient expression of IEX-1 in cells under a variety of stress conditions. IEX-1 is expressed as both a longer form (IEX1L) and a splice variant, designated IEX1S. It is localized to the nucleus and perinuclear region. Overexpression of IEX-1 facilitates apoptosis and cell cycle progression, whereas disruption of IEX-1 expression is associated with decreases in both apoptosis and cell cycle progression.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IEX1 antibody can be used for detection of IEX1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of IEX-1 in mouse skin tissue lysate with IEX-1 antibody at (A) 1 and (B) 2 μg/mL..
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Immunohistochemistry of IEX-1 in human liver tissue with IEX-1 antibody at 2.5 μg/mL.
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Immunofluorescence of IEX-1 in Human Brain cells with IEX-1 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For IER3 (Source: Uniprot.org, NCBI)
Gene Name
IER3
Full Name
Radiation-inducible immediate-early gene IEX-1
Weight
16.903kDa
Superfamily
IER3 family
Alternative Names
DIF-2; DIF2GLY96; Differentiation-dependent gene 2 protein; expressed in pancreatic carcinoma; gly96, mouse, homolog of; IEX-1; IEX1death protein; IEX-1L; Immediate early protein GLY96; Immediate early response 3 protein; immediate early response 3; immediately early gene X-1; PRG1PACAP-responsive gene 1 protein; Protein DIF-2; Protein PRG1; radiation-inducible immediate-early gene IEX-1 IER3 DIF-2, DIF2, GLY96, IEX-1, IEX-1L, IEX1, PRG1 immediate early response 3 radiation-inducible immediate-early gene IEX-1|PACAP-responsive gene 1 protein|anti-death protein|differentiation-dependent gene 2 protein|expressed in pancreatic carcinoma|gly96, mouse, homolog of|immediate early protein GLY96|immediate early response 3 protein|immediately early gene X-1|protein DIF-2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IER3, check out the IER3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IER3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IEX-1 IER3 Antibody (A04014)
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