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Product Info Summary
| SKU: | M05183 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-IGHD Monoclonal Antibody
SKU/Catalog Number
M05183
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-IGHD Monoclonal Antibody catalog # M05183. Tested in WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IGHD Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05183)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AFBO-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human IGHD There are two named isoforms of IGHD produced by alternative splicing, a secreted form and a single pass type I membrane protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M05183 is reactive to IGHD in Human
Applications
M05183 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
14 kDa
Calculated molecular weight
42.353kDa
Background of IgD
Putative transcription factor involved in pancreas development and function.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:500
Validation Images & Assay Conditions
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Western blot analysis of IGHD expression in Humantonsil cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For IGHD (Source: Uniprot.org, NCBI)
Gene Name
IGHD
Full Name
Immunoglobulin heavy constant delta
Weight
42.353kDa
Alternative Names
IgD; Igh-5; Ighd; Immunoglobulin D; Immunoglobulin Delta IGHD immunoglobulin heavy constant delta constant region of heavy chain of IgD
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IGHD, check out the IGHD Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IGHD: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IGHD Monoclonal Antibody (M05183)
Hello CJ!
No publications found for M05183
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
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Customer Reviews
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*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
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Find answers in Q&As, reviews.
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4 Customer Q&As for Anti-IGHD Monoclonal Antibody
Question
Does anti-IGHD Monoclonal antibody M05183 work on zebrafish WB with b-cell?
Verified Customer
Verified customer
Asked: 2019-12-19
Answer
Our lab technicians have not validated anti-IGHD Monoclonal antibody M05183 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-IGHD Monoclonal antibody M05183 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish b-cell in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-19
Question
I was wanting to use your anti-IGHD Monoclonal antibody for WB for human trachea on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human trachea identification?
Verified Customer
Verified customer
Asked: 2018-10-08
Answer
You can see on the product datasheet, M05183 anti-IGHD Monoclonal antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human trachea in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-10-08
Question
I see that the anti-IGHD Monoclonal antibody M05183 works with WB, what is the protocol used to produce the result images on the product page?
A. Yang
Verified customer
Asked: 2017-02-09
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-02-09
Question
My question regarding product M05183, anti-IGHD Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
R. Mitchell
Verified customer
Asked: 2015-09-15
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M05183 anti-IGHD Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-09-15
