Anti-IKK epsilon IKBKE Antibody

Product Name

Anti-IKK epsilon IKBKE Antibody

View all IKK epsilon/IKBKE Antibodies

SKU/Catalog Number

A01816

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-IKK epsilon IKBKE Antibody (Catalog # A01816). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

IKK epsilon antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-IKK epsilon IKBKE Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01816)

Host

Rabbit

Contents

IKK epsilon Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Anti-IKK epsilon antibody was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human IKK epsilon. The immunogen is located within the last 50 amino acids of IKK epsilon.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

It has no cross response to IKKa, IKKb, or IKKy.

Reactive Species

A01816 is reactive to IKBKE in Human, Mouse

Applications

A01816 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

80.462kDa

Background of IKK epsilon/IKBKE

Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-κB is associated with IκB proteins in the cell cytoplasm, which inhibit NF-κB activity. IκB is phosphorylated by IκB kinase (IKK) complex that contains IKKα, IKKβ, and IKKγ. A novel molecule in the IKK complex was recently identified and designated IKKε/IKK-i. IKK epsilon is required for the activation of NF-κB by PMA and T cell receptors but not by TNFα and IL-1. IKKε/IKK-i message is expressed in a variety of tissues and is inducible by TNFα, IL-1, and LPS.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1-2 μg/mL; IHC: 10 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Induction Validation in Mouse Cell Line
Loading: 15 μg of Raw264.7 cell lysates per lane.Antibodies: IKK epsilon A01816 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Cells were treated with LPS (0.3 μg/mL) for 3 hrs (lane 2) and 6 hrs (lane 3) or with control (lane 1).

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Western Blot Validation in Human and Mouse Cell Lines
Loading: 15 μg of lysates per lane.Antibodies: IKK epsilon A01816 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

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Western Blot Validation in Mouse Tissue Lysates
Loading: 15 μg of lysates per lane. Antibodies: IKK epsilon A01816 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

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Western Blot Validation in Jurkat Cell Lysate
Loading: 15 μg of lysates per lane. Antibodies: IKK epsilon A01816 (2 μg/mL)

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Immunohistochemistry Validation of IKK epsilon in Human Pancreas Tissue
Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-IKK epsilon antibody (A01816) at 10 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

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Immunofluorescence Validation of IKK epsilon in HeLa Cells
Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling IKK epsilon with A01816 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Gene/Protein Information For IKBKE (Source: Uniprot.org, NCBI)

Alternative Names

EC 2.7.11; EC 2.7.11.10; I-kappa-B kinase epsilon; IKBKE; IKK epsilon; IKK-E; IKKEMGC125297; IKK-epsilon; IKKI; IKK-I; IKK-iMGC125295; Inducible I kappa-B kinase; inducible IkappaB kinase; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon; inhibitor of nuclear factor kappa-B kinase subunit epsilon; KIAA0151IKK-related kinase epsilon; MGC125294 IKBKE IKK-E, IKK-i, IKKE, IKKI inhibitor of nuclear factor kappa B kinase subunit epsilon inhibitor of nuclear factor kappa-B kinase subunit epsilon|I-kappa-B kinase epsilon|IKK-epsilon|IKK-related kinase epsilon|inducible I kappa-B kinase|inducible IkappaB kinase|inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon

For more info on IKBKE, check out the IKBKE Infographic

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In this infographic, you will see the following information for IKBKE: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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