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Product Info Summary
| SKU: | M00468 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Integrin alpha 4 ITGA4 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00468
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Integrin alpha 4 ITGA4 Rabbit Monoclonal Antibody catalog # M00468. Tested in WB application. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Integrin alpha 4 ITGA4 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00468)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
IHH-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Integrin alpha 4
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00468 is reactive to ITGA4 in Human
Applications
M00468 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
24 kDa, 29 kDa
Calculated molecular weight
114.9kDa
Background of ITGA4
C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
Validation Images & Assay Conditions
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Western blot analysis of Integrin alpha 4 expression in Jurkat cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For ITGA4 (Source: Uniprot.org, NCBI)
Gene Name
ITGA4
Full Name
Integrin alpha-4
Weight
114.9kDa
Superfamily
integrin alpha chain family
Alternative Names
269C wild type; CD49 antigen-like family member D; CD49d antigen; CD49d; CD49Dantigen CD49D, alpha-4 subunit of VLA-4 receptor; IA4; Integrin alpha 4; integrin alpha-4 subunit; integrin alpha-4; Integrin alpha-IV; integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor); ITGA4; MGC90518; very late activation protein 4 receptor, alpha 4 subunit; VLA-4 subunit alpha; VLA-4 ITGA4 CD49D, IA4 integrin subunit alpha 4 integrin alpha-4|269C wild type|CD49 antigen-like family member D|VLA-4 subunit alpha|alpha 4 subunit of VLA-4 receptor|antigen CD49D, alpha-4 subunit of VLA-4 receptor|integrin alpha-IV|very late activation protein 4 receptor, alpha 4 subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ITGA4, check out the ITGA4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ITGA4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Integrin alpha 4 ITGA4 Rabbit Monoclonal Antibody (M00468)
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No publications found for M00468
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Integrin alpha 4 ITGA4 Rabbit Monoclonal Antibody
Question
Will anti-Integrin alpha 4 Rabbit Monoclonal antibody M00468 work for WB with prostate?
J. Parker
Verified customer
Asked: 2019-11-01
Answer
According to the expression profile of prostate, ITGA4 is highly expressed in prostate. So, it is likely that anti-Integrin alpha 4 Rabbit Monoclonal antibody M00468 will work for WB with prostate.
Boster Scientific Support
Answered: 2019-11-01
Question
My question regarding product M00468, anti-Integrin alpha 4 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-12-11
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00468 anti-Integrin alpha 4 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-12-11
Question
Is this M00468 anti-Integrin alpha 4 Rabbit Monoclonal antibody reactive to the isotypes of ITGA4?
G. Brown
Verified customer
Asked: 2018-11-20
Answer
The immunogen of M00468 anti-Integrin alpha 4 Rabbit Monoclonal antibody is A synthesized peptide derived from human Integrin alpha 4. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-11-20
Question
I am interested in to test anti-Integrin alpha 4 Rabbit Monoclonal antibody M00468 on human prostate for research purposes, then I may be interested in using anti-Integrin alpha 4 Rabbit Monoclonal antibody M00468 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-06-19
Answer
The products we sell, including anti-Integrin alpha 4 Rabbit Monoclonal antibody M00468, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-06-19
Question
Do you have a BSA free version of anti-Integrin alpha 4 Rabbit Monoclonal antibody M00468 available?
H. Edwards
Verified customer
Asked: 2017-12-29
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Integrin alpha 4 Rabbit Monoclonal antibody M00468 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-12-29
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for prostate using anti-Integrin alpha 4 Rabbit Monoclonal antibody M00468. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-10-10
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-10-10
Question
I was wanting to use your anti-Integrin alpha 4 Rabbit Monoclonal antibody for WB for human prostate on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human prostate identification?
D. Yang
Verified customer
Asked: 2017-04-21
Answer
You can see on the product datasheet, M00468 anti-Integrin alpha 4 Rabbit Monoclonal antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human prostate in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-04-21
