Anti-Integrin alpha 6 ITGA6 Rabbit Monoclonal Antibody

Product Name

Anti-Integrin alpha 6 ITGA6 Rabbit Monoclonal Antibody

View all Integrin alpha 6/CD49f Antibodies

SKU/Catalog Number

M01693

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Integrin alpha 6 ITGA6 Rabbit Monoclonal Antibody catalog # M01693. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Integrin alpha 6 ITGA6 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01693)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

DHG-9

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Integrin alpha 6

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01693 is reactive to ITGA6 in Human, Mouse, Rat

Applications

M01693 is guaranteed for IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

v

Calculated molecular weight

126.606kDa

Background of Integrin alpha 6/CD49f

Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX (K/R) R consensus motif.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:100
ICC/IF 1:50-1:200

Validation Images & Assay Conditions

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Western blot analysis of Integrin alpha 6 expression in SW480 cell lysate (M01693).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITGA6 monoclonal antibody (Catalog # M01693) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ITGA6

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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature

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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature

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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.

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Immunohistochemical analysis of paraffin-embedded human uterus, using Integrin alpha 6 Antibody(M01693)
ITGA6 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ITGA6 Antibody (M01693)overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

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Figure 3. IF analysis of ITGA6 using anti-ITGA6 antibody (M01693).
ITGA6 was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated at 1:50 with rabbit anti-ITGA6 Antibody (M01693) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Gene/Protein Information For ITGA6 (Source: Uniprot.org, NCBI)

Alternative Names

CD49 antigen-like family member F; CD49f antigen; CD49f; DKFZp686J01244; FLJ18737; Integrin alpha 6; integrin alpha-6; integrin alpha6B; integrin, alpha 6; ITGA6; ITGA6B; Platelet gpl; VLA-6 ITGA6 CD49fB, VLA-6, ITGA6 integrin subunit alpha 6 integrin alpha-6|CD49 antigen-like family member F|integrin alpha6B|integrin, alpha 6

For more info on ITGA6, check out the ITGA6 Infographic

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In this infographic, you will see the following information for ITGA6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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