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Product Info Summary
| SKU: | M00278 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-IP10 CXCL10 Rabbit Monoclonal Antibody
View all CXCL10/IP-10/CRG-2 Antibodies
SKU/Catalog Number
M00278
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-IP10 CXCL10 Rabbit Monoclonal Antibody catalog # M00278. Tested in WB application. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IP10 CXCL10 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00278)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AACI-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human IP10
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00278 is reactive to CXCL10 in Human
Applications
M00278 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
75 kDa
Calculated molecular weight
10.881kDa
Background of CXCL10/IP-10/CRG-2
C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of IP10 expression in IP10 recombinant protein lysate.
Protein Target Info & Infographic
Gene/Protein Information For CXCL10 (Source: Uniprot.org, NCBI)
Gene Name
CXCL10
Full Name
C-X-C motif chemokine 10
Weight
10.881kDa
Superfamily
intercrine alpha (chemokine CxC) family
Alternative Names
C7; chemokine (C-X-C motif) ligand 10; CRG2; CRG-2; CXCL10; gIP-10; IFI10; INP10; IP-10; mob-1; SCYB10 CXCL10 C7, IFI10, INP10, IP-10, SCYB10, crg-2, gIP-10, mob-1 C-X-C motif chemokine ligand 10 C-X-C motif chemokine 10|10 kDa interferon gamma-induced protein|gamma IP10|interferon-inducible cytokine IP-10|protein 10 from interferon (gamma)-induced cell line|small inducible cytokine subfamily B (Cys-X-Cys), member 10|small-inducible cytokine B10
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CXCL10, check out the CXCL10 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CXCL10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IP10 CXCL10 Rabbit Monoclonal Antibody (M00278)
Hello CJ!
M00278 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
A correlation study between gene polymorphism of Th cell expressed chemokine receptor CXCR3 and its ligand levels with HCV infection prognosis
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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14 Customer Q&As for Anti-IP10 CXCL10 Rabbit Monoclonal Antibody
Question
Is this M00278 anti-IP10 Rabbit Monoclonal antibody reactive to the isotypes of CXCL10?
Verified Customer
Verified customer
Asked: 2020-04-14
Answer
The immunogen of M00278 anti-IP10 Rabbit Monoclonal antibody is A synthesized peptide derived from human IP10. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-14
Question
I was wanting to use your anti-IP10 Rabbit Monoclonal antibody for WB for human prostate on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human prostate identification?
Verified Customer
Verified customer
Asked: 2020-03-25
Answer
You can see on the product datasheet, M00278 anti-IP10 Rabbit Monoclonal antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human prostate in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-25
Question
My question regarding product M00278, anti-IP10 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00278 anti-IP10 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-04
Question
Here is the WB image, lot number and protocol we used for prostate using anti-IP10 Rabbit Monoclonal antibody M00278. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-04
Question
I see that the anti-IP10 Rabbit Monoclonal antibody M00278 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-01
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-01-01
Question
Is a blocking peptide available for product anti-IP10 Rabbit Monoclonal antibody (M00278)?
B. Wu
Verified customer
Asked: 2019-08-16
Answer
We do provide the blocking peptide for product anti-IP10 Rabbit Monoclonal antibody (M00278). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-08-16
Question
Would anti-IP10 Rabbit Monoclonal antibody M00278 work for WB with prostate?
Verified Customer
Verified customer
Asked: 2019-08-15
Answer
According to the expression profile of prostate, CXCL10 is highly expressed in prostate. So, it is likely that anti-IP10 Rabbit Monoclonal antibody M00278 will work for WB with prostate.
Boster Scientific Support
Answered: 2019-08-15
Question
I am looking for to test anti-IP10 Rabbit Monoclonal antibody M00278 on human prostate for research purposes, then I may be interested in using anti-IP10 Rabbit Monoclonal antibody M00278 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-11-29
Answer
The products we sell, including anti-IP10 Rabbit Monoclonal antibody M00278, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-11-29
Question
Is there a BSA free version of anti-IP10 Rabbit Monoclonal antibody M00278 available?
Verified Customer
Verified customer
Asked: 2018-09-24
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IP10 Rabbit Monoclonal antibody M00278 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-09-24
Question
We are currently using anti-IP10 Rabbit Monoclonal antibody M00278 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2018-05-03
Answer
The anti-IP10 Rabbit Monoclonal antibody (M00278) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-05-03
Question
Our team were well pleased with the WB result of your anti-IP10 Rabbit Monoclonal antibody. However we have seen positive staining in ectocervix secreted. using this antibody. Is that expected? Could you tell me where is CXCL10 supposed to be expressed?
B. Williams
Verified customer
Asked: 2017-07-06
Answer
From literature, ectocervix does express CXCL10. Generally CXCL10 expresses in secreted. Regarding which tissues have CXCL10 expression, here are a few articles citing expression in various tissues:
Foreskin keratinocyte, Pubmed ID: 11559369
Keratinocyte, Pubmed ID: 10233762
Prostate, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-07-06
Question
We have observed staining in human prostate. What should we do? Is anti-IP10 Rabbit Monoclonal antibody supposed to stain prostate positively?
Verified Customer
Verified customer
Asked: 2017-05-18
Answer
From what I have seen in literature prostate does express CXCL10. From what I have seen in Uniprot.org, CXCL10 is expressed in ectocervix, prostate, keratinocyte, foreskin keratinocyte, among other tissues. Regarding which tissues have CXCL10 expression, here are a few articles citing expression in various tissues:
Foreskin keratinocyte, Pubmed ID: 11559369
Keratinocyte, Pubmed ID: 10233762
Prostate, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-05-18
Question
Will M00278 anti-IP10 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
O. Moore
Verified customer
Asked: 2016-03-25
Answer
You can see on the product datasheet, M00278 anti-IP10 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-03-25
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for prostate using anti-IP10 Rabbit Monoclonal antibody M00278. Let me know if you need anything else.
E. Williams
Verified customer
Asked: 2013-08-21
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-08-21
