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Product Info Summary
| SKU: | M00115 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-IRF7 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00115
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-IRF7 Rabbit Monoclonal Antibody catalog # M00115. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IRF7 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00115)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
FAH-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human IRF7
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00115 is reactive to IRF7 in Human, Mouse, Rat
Applications
M00115 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
31 kDa
Calculated molecular weight
54.278kDa
Background of IRF7
Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns (4,5) P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) . PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human pancreas, using IRF7 Antibody.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of IRF7 expression in Jurkat cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For IRF7 (Source: Uniprot.org, NCBI)
Gene Name
IRF7
Full Name
Interferon regulatory factor 7
Weight
54.278kDa
Superfamily
IRF family
Alternative Names
interferon regulatory factor 7; interferon regulatory factor-7H; IRF-7; IRF7A; IRF-7H IRF7 IMD39, IRF-7, IRF-7HA, IRF7B, IRF7C, IRF7H, IRF7 interferon regulatory factor 7 interferon regulatory factor 7|interferon regulatory factor-7H
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IRF7, check out the IRF7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IRF7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IRF7 Rabbit Monoclonal Antibody (M00115)
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No publications found for M00115
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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17 Customer Q&As for Anti-IRF7 Rabbit Monoclonal Antibody
Question
My colleagues were happy with the WB result of your anti-IRF7 Rabbit Monoclonal antibody. However we have seen positive staining in spleen nucleus. cytoplasm. note=the phosphorylated using this antibody. Is that expected? Could you tell me where is IRF7 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-04-28
Answer
From literature, spleen does express IRF7. Generally IRF7 expresses in nucleus. cytoplasm. note=the phosphorylated. Regarding which tissues have IRF7 expression, here are a few articles citing expression in various tissues:
Spleen, Pubmed ID: 9315633
Boster Scientific Support
Answered: 2020-04-28
Question
I was wanting to use your anti-IRF7 Rabbit Monoclonal antibody for IP for human right lobe of liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human right lobe of liver identification?
Verified Customer
Verified customer
Asked: 2020-04-21
Answer
As indicated on the product datasheet, M00115 anti-IRF7 Rabbit Monoclonal antibody has been tested for Flow Cytometry, IP, IF, IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human right lobe of liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-21
Question
I see that the anti-IRF7 Rabbit Monoclonal antibody M00115 works with IP, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-04-13
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-04-13
Question
Is a blocking peptide available for product anti-IRF7 Rabbit Monoclonal antibody (M00115)?
Verified Customer
Verified customer
Asked: 2020-02-05
Answer
We do provide the blocking peptide for product anti-IRF7 Rabbit Monoclonal antibody (M00115). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-02-05
Question
Does anti-IRF7 Rabbit Monoclonal antibody M00115 work on primate WB with spleen?
Verified Customer
Verified customer
Asked: 2020-01-24
Answer
Our lab technicians have not tested anti-IRF7 Rabbit Monoclonal antibody M00115 on primate. You can run a BLAST between primate and the immunogen sequence of anti-IRF7 Rabbit Monoclonal antibody M00115 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate spleen in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-24
Question
Can you help my question with product M00115, anti-IRF7 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-02
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00115 anti-IRF7 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-02
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for right lobe of liver using anti-IRF7 Rabbit Monoclonal antibody M00115. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-29
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-29
Question
We have been able to see staining in rat right lobe of liver. Any tips? Is anti-IRF7 Rabbit Monoclonal antibody supposed to stain right lobe of liver positively?
Verified Customer
Verified customer
Asked: 2019-04-16
Answer
From literature right lobe of liver does express IRF7. From Uniprot.org, IRF7 is expressed in right lobe of liver, spleen, among other tissues. Regarding which tissues have IRF7 expression, here are a few articles citing expression in various tissues:
Spleen, Pubmed ID: 9315633
Boster Scientific Support
Answered: 2019-04-16
Question
I am interested in to test anti-IRF7 Rabbit Monoclonal antibody M00115 on human right lobe of liver for research purposes, then I may be interested in using anti-IRF7 Rabbit Monoclonal antibody M00115 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-01-16
Answer
The products we sell, including anti-IRF7 Rabbit Monoclonal antibody M00115, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-01-16
Question
Is this M00115 anti-IRF7 Rabbit Monoclonal antibody reactive to the isotypes of IRF7?
Verified Customer
Verified customer
Asked: 2018-10-08
Answer
The immunogen of M00115 anti-IRF7 Rabbit Monoclonal antibody is A synthesized peptide derived from human IRF7. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-10-08
Question
you antibody using your anti-IRF7 Rabbit Monoclonal antibody for regulation of monocyte differentiation studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.
B. Mangal
Verified customer
Asked: 2017-01-27
Answer
We appreciate your inquiry. This M00115 anti-IRF7 Rabbit Monoclonal antibody is tested on jurkat cell lysate. It is guaranteed to work for Flow Cytometry, IP, IF, IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-01-27
Question
We are currently using anti-IRF7 Rabbit Monoclonal antibody M00115 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?
B. Gonzalez
Verified customer
Asked: 2016-11-09
Answer
The anti-IRF7 Rabbit Monoclonal antibody (M00115) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-11-09
Question
See attached the WB image, lot number and protocol we used for right lobe of liver using anti-IRF7 Rabbit Monoclonal antibody M00115. Please let me know if you require anything else.
M. Wu
Verified customer
Asked: 2016-02-11
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-02-11
Question
Does anti-IRF7 Rabbit Monoclonal antibody M00115 work for IP with right lobe of liver?
N. Brown
Verified customer
Asked: 2015-12-25
Answer
According to the expression profile of right lobe of liver, IRF7 is highly expressed in right lobe of liver. So, it is likely that anti-IRF7 Rabbit Monoclonal antibody M00115 will work for IP with right lobe of liver.
Boster Scientific Support
Answered: 2015-12-25
Question
Is there a BSA free version of anti-IRF7 Rabbit Monoclonal antibody M00115 available?
M. Jones
Verified customer
Asked: 2015-09-10
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-IRF7 Rabbit Monoclonal antibody M00115 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-09-10
Question
We ordered your anti-IRF7 Rabbit Monoclonal antibody for Flow Cytometry on spleen in a previous project. I am using mouse, and We intend to use the antibody for ICC next. I am looking for examining spleen as well as right lobe of liver in our next experiment. Could you please give me some suggestion on which antibody would work the best for ICC?
K. Singh
Verified customer
Asked: 2015-04-27
Answer
I have checked the website and datasheets of our anti-IRF7 Rabbit Monoclonal antibody and it appears that M00115 has been validated on mouse in both Flow Cytometry and ICC. Thus M00115 should work for your application. Our Boster satisfaction guarantee will cover this product for ICC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for ICC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2015-04-27
Question
Does M00115 anti-IRF7 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
O. Jones
Verified customer
Asked: 2013-03-12
Answer
As indicated on the product datasheet, M00115 anti-IRF7 Rabbit Monoclonal antibody as been validated on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-03-12
