Anti-Junctophilin-1 JPH1 Antibody

Product Name

Anti-Junctophilin-1 JPH1 Antibody

View all JPH1 Antibodies

SKU/Catalog Number

A08565

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Junctophilin-1 JPH1 Antibody (Catalog # A08565). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

JPH1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Junctophilin-1 JPH1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08565)

Host

Rabbit

Contents

JPH1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

JPH1 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human JPH1. The immunogen is located within amino acids 470 - 520 of JPH1.

Reactive Species

A08565 is reactive to JPH1 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

71.7 kDa

Background of JPH1

Junctional complexes between the plasma membrane (PM) and endoplasmic/sarcoplasmic reticulum (ER/SR) are a common feature of all excitable cell types and mediate cross talk between cell surface and intracellular ion channels. Junctophilins (JPs) are important components of the junctional complexes. JPs are composed of a carboxy-terminal hydrophobic segment spanning the ER/SR membrane and a remaining cytoplasmic domain that shows specific affinity for the PM. Four JPs have been identified as tissue-specific subtypes derived from different genes: JPH1 is expressed in skeletal muscle, JPH2 is detected throughout all muscle cell types, and JPH3 and JPH4 are predominantly expressed in the brain and contribute to the subsurface cistern formation in neurons. JPH1 is essential for stabilizing the T-tubule and SR membranes to form junctions and provide an environment for the assembly of receptors such as the ryanodine receptor type 1 (RyR1).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A08565 is guaranteed for ELISA, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

JPH1 antibody can be used for detection of JPH1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information JPH1

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