Product Info Summary
SKU: | M02848-1 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-K48-linkage Specific Ubiquitin UBB Rabbit Monoclonal Antibody
SKU/Catalog Number
M02848-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-K48-linkage Specific Ubiquitin UBB Rabbit Monoclonal Antibody catalog # M02848-1. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-K48-linkage Specific Ubiquitin UBB Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02848-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
IBH-21
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human K48-linkage Specific Ubiquitin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
Reactive Species
M02848-1 is reactive to UBB in Human, Mouse, Rat
Applications
M02848-1 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
55 kDa
Calculated molecular weight
25.762kDa
Background of UBB
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:100-1:500
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Ubiquitin expression in Jurkat cell lysate.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human endometrium carcinoma, using K48-linkage Specific Ubiquitin Antibody.
Click image to see more details
Immunofluorescent analysis of MCF-7 cells, using K48-linkage Specific Ubiquitin Antibody.
Protein Target Info & Infographic
Gene/Protein Information For UBB (Source: Uniprot.org, NCBI)
Gene Name
UBB
Full Name
Polyubiquitin-B
Weight
25.762kDa
Superfamily
ubiquitin family
Alternative Names
RPS27A; UBA52; UBB ubiquitin B; UBB; UBC; Ubiquitin UBB HEL-S-50 ubiquitin B polyubiquitin-B|epididymis secretory protein Li 50|polyubiquitin B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on UBB, check out the UBB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for UBB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-K48-linkage Specific Ubiquitin UBB Rabbit Monoclonal Antibody (M02848-1)
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No publications found for M02848-1
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-K48-linkage Specific Ubiquitin UBB Rabbit Monoclonal Antibody
Question
Would anti-K48-linkage Specific Ubiquitin Rabbit Monoclonal antibody M02848-1 work for ICC with fetal brain cortex?
Verified Customer
Verified customer
Asked: 2020-03-23
Answer
According to the expression profile of fetal brain cortex, UBB is highly expressed in fetal brain cortex. So, it is likely that anti-K48-linkage Specific Ubiquitin Rabbit Monoclonal antibody M02848-1 will work for ICC with fetal brain cortex.
Boster Scientific Support
Answered: 2020-03-23
Question
Our lab used your anti-K48-linkage Specific Ubiquitin Rabbit Monoclonal antibody for IF on fetal brain cortex in the past. I am using human, and We want to use the antibody for IHC next. We want examining fetal brain cortex as well as blood in our next experiment. Could give a recommendation on which antibody would work the best for IHC?
M. Edwards
Verified customer
Asked: 2020-03-17
Answer
I viewed the website and datasheets of our anti-K48-linkage Specific Ubiquitin Rabbit Monoclonal antibody and it appears that M02848-1 has been validated on human in both IF and IHC. Thus M02848-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-03-17
Question
My lab would like to test anti-K48-linkage Specific Ubiquitin Rabbit Monoclonal antibody M02848-1 on mouse fetal brain cortex for research purposes, then I may be interested in using anti-K48-linkage Specific Ubiquitin Rabbit Monoclonal antibody M02848-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
The products we sell, including anti-K48-linkage Specific Ubiquitin Rabbit Monoclonal antibody M02848-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-04
Question
Is there a BSA free version of anti-K48-linkage Specific Ubiquitin Rabbit Monoclonal antibody M02848-1 available?
Verified Customer
Verified customer
Asked: 2019-12-23
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-K48-linkage Specific Ubiquitin Rabbit Monoclonal antibody M02848-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-23
Question
We were well pleased with the WB result of your anti-K48-linkage Specific Ubiquitin Rabbit Monoclonal antibody. However we have been able to see positive staining in brain ubiquitin: cytoplasm. nucleus using this antibody. Is that expected? Could you tell me where is UBB supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-12-23
Answer
Based on literature, brain does express UBB. Generally UBB expresses in ubiquitin: cytoplasm. nucleus. Regarding which tissues have UBB expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 3029682
Brain, Liver, and Lung, Pubmed ID: 15489334
Fetal brain cortex, Pubmed ID: 1128706
Boster Scientific Support
Answered: 2019-12-23
Question
Will M02848-1 anti-K48-linkage Specific Ubiquitin Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-05
Answer
It shows on the product datasheet, M02848-1 anti-K48-linkage Specific Ubiquitin Rabbit Monoclonal antibody as been tested on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-05
Question
We want using your anti-K48-linkage Specific Ubiquitin Rabbit Monoclonal antibody for negative regulation of mapk pathway studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-08-16
Answer
We appreciate your inquiry. This M02848-1 anti-K48-linkage Specific Ubiquitin Rabbit Monoclonal antibody is validated on jurkat cell lysate. It is guaranteed to work for Flow Cytometry, IF, IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-08-16
Question
Is this M02848-1 anti-K48-linkage Specific Ubiquitin Rabbit Monoclonal antibody reactive to the isotypes of UBB?
Verified Customer
Verified customer
Asked: 2019-07-15
Answer
The immunogen of M02848-1 anti-K48-linkage Specific Ubiquitin Rabbit Monoclonal antibody is A synthesized peptide derived from human K48-linkage Specific Ubiquitin. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-15
Question
We have been able to see staining in rat hypothalamus. What should we do? Is anti-K48-linkage Specific Ubiquitin Rabbit Monoclonal antibody supposed to stain hypothalamus positively?
Verified Customer
Verified customer
Asked: 2019-05-31
Answer
According to literature hypothalamus does express UBB. According to Uniprot.org, UBB is expressed in hypothalamus, blood, brain, liver lung, fetal brain cortex, among other tissues. Regarding which tissues have UBB expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 3029682
Brain, Liver, and Lung, Pubmed ID: 15489334
Fetal brain cortex, Pubmed ID: 1128706
Boster Scientific Support
Answered: 2019-05-31
Question
See attached the WB image, lot number and protocol we used for fetal brain cortex using anti-K48-linkage Specific Ubiquitin Rabbit Monoclonal antibody M02848-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-05-30
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-30
Question
My question regarding product M02848-1, anti-K48-linkage Specific Ubiquitin Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
S. Patel
Verified customer
Asked: 2018-05-14
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02848-1 anti-K48-linkage Specific Ubiquitin Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-05-14
Question
I was wanting to use your anti-K48-linkage Specific Ubiquitin Rabbit Monoclonal antibody for ICC for mouse fetal brain cortex on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse fetal brain cortex identification?
K. Jones
Verified customer
Asked: 2017-11-23
Answer
You can see on the product datasheet, M02848-1 anti-K48-linkage Specific Ubiquitin Rabbit Monoclonal antibody has been tested for Flow Cytometry, IF, IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse fetal brain cortex in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-11-23
Question
Is a blocking peptide available for product anti-K48-linkage Specific Ubiquitin Rabbit Monoclonal antibody (M02848-1)?
Verified Customer
Verified customer
Asked: 2017-08-24
Answer
We do provide the blocking peptide for product anti-K48-linkage Specific Ubiquitin Rabbit Monoclonal antibody (M02848-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-08-24
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for fetal brain cortex using anti-K48-linkage Specific Ubiquitin Rabbit Monoclonal antibody M02848-1. Let me know if you need anything else.
C. Li
Verified customer
Asked: 2015-05-04
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-05-04
Question
We are currently using anti-K48-linkage Specific Ubiquitin Rabbit Monoclonal antibody M02848-1 for mouse tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
E. Parker
Verified customer
Asked: 2013-08-06
Answer
The anti-K48-linkage Specific Ubiquitin Rabbit Monoclonal antibody (M02848-1) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-08-06
Question
I see that the anti-K48-linkage Specific Ubiquitin Rabbit Monoclonal antibody M02848-1 works with ICC, what is the protocol used to produce the result images on the product page?
M. Parker
Verified customer
Asked: 2013-07-19
Answer
You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-07-19