Product Info Summary
SKU: | A04321-3 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-KIF7 Antibody Picoband™
SKU/Catalog Number
A04321-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-KIF7 Antibody Picoband™ catalog # A04321-3. Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-KIF7 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04321-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human KIF7, identical to the related mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04321-3 is reactive to Kif7 in Human
Applications
A04321-3 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
151 kDa
Calculated molecular weight
151.624kDa
Background of Kif7
This gene encodes a cilia-associated protein belonging to the kinesin family. This protein plays a role in the sonic hedgehog (SHH) signaling pathway through the regulation of GLI transcription factors. It functions as a negative regulator of the SHH pathway by preventing inappropriate activation of GLI2 in the absence of ligand, and as a positive regulator by preventing the processing of GLI3 into its repressor form. Mutations in this gene have been associated with various ciliopathies.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of KIF7 using anti-KIF7 antibody (A04321-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human A375 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human Jurkat whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KIF7 antigen affinity purified polyclonal antibody (Catalog # A04321-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KIF7 at approximately 151 kDa. The expected band size for KIF7 is at 151 kDa.
Protein Target Info & Infographic
Gene/Protein Information For Kif7 (Source: Uniprot.org, NCBI)
Gene Name
Kif7
Full Name
Kinesin-like protein KIF7
Weight
151.624kDa
Superfamily
TRAFAC class myosin-kinesin ATPase superfamily
Alternative Names
EQYK340; kinesin family member 7; kinesin-like protein KIF7; MGC120653; MGC138476; MGC138478; UNQ340 Kif7|kinesin family member 7|kinesin-like protein KIF7|kinesin 7
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Kif7, check out the Kif7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Kif7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-KIF7 Antibody Picoband™ (A04321-3)
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No publications found for A04321-3
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Customer Reviews
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