Product Info Summary
SKU: | A30483 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-KLF Antibody
SKU/Catalog Number
A30483
Size
100ul
Form
Liquid
Description
Boster Bio Anti-KLF Antibody (Catalog# A30483). Tested in WB, IF, ICC, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-KLF Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30483)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human KLF. Immunogen sequence location: 291-340
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A30483 is reactive to KLF1 in Human, Mouse, Rat
Applications
A30483 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
72 kDa
Calculated molecular weight
38.221kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:20000
Not yet tested in other applications.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of lysates from Jurkat cells, treated with serum 20% 15', using KLF Antibody. The lane on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For KLF1 (Source: Uniprot.org, NCBI)
Gene Name
KLF1
Full Name
Krueppel-like factor 1
Weight
38.221kDa
Superfamily
krueppel C2H2-type zinc-finger protein family
Alternative Names
EKLFerythroid-specific transcription factor EKLF; Erythroid krueppel-like transcription factor; erythroid Kruppel-like factor; HBFQTL6; INLU; Krueppel-like factor 1; Kruppel-like factor 1 (erythroid); monoclonal A3D8 KLF1 EKLF, EKLF/KLF1 Kruppel like factor 1 Krueppel-like factor 1|erythroid Kruppel-like factor|erythroid krueppel-like transcription factor|erythroid-specific transcription factor EKLF|truncated Kruppel-like factor 1|truncated erythroid Kruppel-like factor EKLF/KLF1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KLF1, check out the KLF1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KLF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-KLF Antibody (A30483)
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No publications found for A30483
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Customer Reviews
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*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
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Find answers in Q&As, reviews.
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3 Customer Q&As for Anti-KLF Antibody
Question
I was wanting to use your anti-KLF antibody for WB for human liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human liver identification?
J. Parker
Verified customer
Asked: 2019-11-01
Answer
You can see on the product datasheet, A30483 anti-KLF antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-01
Question
Is a blocking peptide available for product anti-KLF antibody (A30483)?
Verified Customer
Verified customer
Asked: 2019-06-11
Answer
We do provide the blocking peptide for product anti-KLF antibody (A30483). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-06-11
Question
We are currently using anti-KLF antibody A30483 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on horse tissues as well?
B. Krishna
Verified customer
Asked: 2014-01-10
Answer
The anti-KLF antibody (A30483) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-01-10