Product Info Summary
SKU: | A00120-2 |
---|---|
Size: | 80 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | Flow Cytometry, IF, WB |
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Product info
Product Name
Anti-KLF4 Antibody (C-term)
SKU/Catalog Number
A00120-2
Size
80 µl
Form
Liquid
Description
Boster Bio Anti-KLF4 Antibody (C-term) (Catalog # A00120-2). Tested in WB, Flow Cytometry, IF application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-KLF4 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00120-2)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This KLF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 394-421 amino acids from the C-terminal region of human KLF4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00120-2 is reactive to KLF4 in Human
Applications
A00120-2 is guaranteed for Flow Cytometry, IF, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
54.671kDa
Background of KLF4
KLF4 is a transcription factor which acts as both an activator and repressor. Binds the CACCC core sequence. Binds to multiple sites in the 5'-flanking region of its own gene and can activate its own transcription. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:10-1:50
WB: 1:1000
FC: 1:10-1:50
Validation Images & Assay Conditions
Click image to see more details
Fluorescent confocal image of SY5Y cells stained with A00120-2 KLF4 (C-term) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with A00120-2 KLF4 (C-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (5.25 μM, 25 min). KLF4 immunoreactivity is localized very specifically to the nuclei of the SY5Y cells.
Click image to see more details
KLF4 Antibody (C-term) western blot analysis in A431,MCF-7 cell line lysates (35ug/lane).This demonstrates the KLF4 antibody detected the KLF4 protein (arrow).
Click image to see more details
KLF4 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Protein Target Info & Infographic
Gene/Protein Information For KLF4 (Source: Uniprot.org, NCBI)
Gene Name
KLF4
Full Name
Krueppel-like factor 4
Weight
54.671kDa
Superfamily
krueppel C2H2-type zinc-finger protein family
Alternative Names
Epithelial zinc finger protein EZF; EZF; EZFendothelial Kruppel-like zinc finger protein; GKLFKrueppel-like factor 4; Gut-enriched krueppel-like factor; KLF4; Kruppel-like factor 4 (gut) KLF4 EZF, GKLF Kruppel like factor 4 Krueppel-like factor 4|Kruppel-like factor 4 (gut)|endothelial Kruppel-like zinc finger protein|epithelial zinc finger protein EZF|gut Kruppel-like factor|gut-enriched krueppel-like factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KLF4, check out the KLF4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KLF4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-KLF4 Antibody (C-term) (A00120-2)
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No publications found for A00120-2
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Customer Reviews
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