Product Info Summary
SKU: | M00825 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Lactate Dehydrogenase LDHA Rabbit Monoclonal Antibody
View all Lactate Dehydrogenase A/LDHA Antibodies
SKU/Catalog Number
M00825
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Lactate Dehydrogenase LDHA Rabbit Monoclonal Antibody catalog # M00825. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Lactate Dehydrogenase LDHA Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00825)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
CFE-12
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Lactate Dehydrogenase
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00825 is reactive to LDHA in Human, Mouse, Rat
Applications
M00825 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
95 kDa
Calculated molecular weight
36.689kDa
Background of Lactate Dehydrogenase A/LDHA
Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:100
ICC/IF 1:50-1:100
IP 1:50
FC 1:50
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human colon, using Lactate Dehydrogenase Antibody.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of LDHA expression in (1)Hela cell lysate;(2)Human heart lysate.
Protein Target Info & Infographic
Gene/Protein Information For LDHA (Source: Uniprot.org, NCBI)
Gene Name
LDHA
Full Name
L-lactate dehydrogenase A chain
Weight
36.689kDa
Superfamily
LDH/MDH superfamily
Alternative Names
Cell proliferation-inducing gene 19 protein; EC 1.1.1; EC 1.1.1.27; GSD11; Lactate Dehydrogenase A; lactate dehydrogenase M; LDH muscle subunit; LDH1; LDHA; LDH-A; LDHM; LDH-M; L-lactate dehydrogenase A chain; proliferation-inducing gene 19; Renal carcinoma antigen NY-REN-59 LDHA GSD11, HEL-S-133P, LDHM, PIG19 lactate dehydrogenase A L-lactate dehydrogenase A chain|LDH muscle subunit|LDH-A|LDH-M|cell proliferation-inducing gene 19 protein|epididymis secretory sperm binding protein Li 133P|lactate dehydrogenase M|proliferation-inducing gene 19|renal carcinoma antigen NY-REN-59
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LDHA, check out the LDHA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LDHA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Lactate Dehydrogenase LDHA Rabbit Monoclonal Antibody (M00825)
Hello CJ!
M00825 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Jiang X,Yuan J,Dou Y,Zeng D, Xiao S.Lipopolysaccharide Affects the Proliferation and Glucose Metabolism of Cervical Cancer Cells Through the FRA1/MDM2/p53 Pathway.Int J Med Sci 2021;18(4): 1030-1038.doi:10. 7150/ijms.47360.
Species: Human
M00825 usage in article: APP:WB, SAMPLE:CASKI CELL AND HELA CELL, DILUTION:NA
Chen J,Wu D,Dong Z,Chen A,Liu S.The expression and role of glycolysis-associated molecules in infantile hemangioma.Life Sci.2020 Oct 15;259:118215.doi:10.1016/j.lfs.2020.118215.Epub 2020 Aug 5.PMID:32768579.
Species: Human
M00825 usage in article: APP:IHC, SAMPLE:NORMAL AND TUMOR SKIN TISSUE, DILUTION:1:200
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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4 Customer Q&As for Anti-Lactate Dehydrogenase LDHA Rabbit Monoclonal Antibody
Question
We are currently using anti-Lactate Dehydrogenase Rabbit Monoclonal antibody M00825 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-19
Answer
The anti-Lactate Dehydrogenase Rabbit Monoclonal antibody (M00825) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-19
Question
We bought anti-Lactate Dehydrogenase Rabbit Monoclonal antibody for IHC on bone marrow in the past. I am using human, and I plan to use the antibody for IF next. I am interested in examining bone marrow as well as b-cell lymphoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2017-12-29
Answer
I have checked the website and datasheets of our anti-Lactate Dehydrogenase Rabbit Monoclonal antibody and I see that M00825 has been tested on human in both IHC and IF. Thus M00825 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-12-29
Question
We were satisfied with the WB result of your anti-Lactate Dehydrogenase Rabbit Monoclonal antibody. However we have been able to see positive staining in renal cell carcinoma cytoplasm. using this antibody. Is that expected? Could you tell me where is LDHA supposed to be expressed?
D. Carter
Verified customer
Asked: 2017-10-23
Answer
From literature, renal cell carcinoma does express LDHA. Generally LDHA expresses in cytoplasm. Regarding which tissues have LDHA expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Lymphoblast, Pubmed ID: 14654843
Umbilical cord, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2017-10-23
Question
We have observed staining in mouse leukemic t-cell. Any tips? Is anti-Lactate Dehydrogenase Rabbit Monoclonal antibody supposed to stain leukemic t-cell positively?
G. Evans
Verified customer
Asked: 2015-06-08
Answer
According to literature leukemic t-cell does express LDHA. According to Uniprot.org, LDHA is expressed in left coronary artery, umbilical cord, gastric carcinoma, bone marrow, b-cell lymphoma, renal cell carcinoma, lymphoblast, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have LDHA expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Lymphoblast, Pubmed ID: 14654843
Umbilical cord, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2015-06-08