Anti-Lamin A + C/LMNA Antibody

Boster Bio Anti-Lamin A + C/LMNA Antibody catalog # PA1103. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 4 publication(s).

Product Info Summary

SKU: PA1103
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, ICC, WB

Product Name

Anti-Lamin A + C/LMNA Antibody

See all LMNA products

SKU/Catalog Number

PA1103

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Lamin A + C/LMNA Antibody catalog # PA1103. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Lamin A + C/LMNA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1103)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Lamin A/C (455-469aa RNKSNEDQSMGNWQI), identical to the related rat and mouse sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA1103 is reactive to LMNA in Human, Mouse, Rat

Applications

PA1103 is guaranteed for IHC, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunocytochemistry , 0.5-1μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For LMNA (Source: Uniprot.Org, NCBI)

Uniprot ID

P02545

Gene ID

4000

Gene Name

LMNA

Full Name

Prelamin-A/C

Weight

74.139kDa

Superfamily

intermediate filament family

Alternative Names

CDCD1; CDDC; CMD1A; CMT2B1; dilated 1A (autosomal dominant); EMD2; FPLD; HGPSFPL; lamin A/C; lamin A/C-like 1; lamin-A/C; LDP1; LFP; LGMD1B; limb girdle muscular dystrophy 1B (autosomal dominant); LMN1IDC; LMNC; LMNL1; prelamin-A/C; PRO1,70 kDa lamin; progeria 1 (Hutchinson-Gilford type); renal carcinoma antigen NY-REN-32 LMNA CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, FPLD2, HGPS, IDC, LDP1, LFP, LGMD1B, LMN1, LMNC, LMNL1, MADA, PRO1 lamin A/C lamin|70 kDa lamin|epididymis secretory sperm binding protein|lamin A/C-like 1|mandibuloacral dysplasia type A|prelamin-A/C|renal carcinoma antigen NY-REN-32

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on LMNA, check out the LMNA Infographic

LMNA infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for LMNA: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PA1103 has been cited in 4 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Li D,Liu J,Yang C,Tian Y,Yin C,Hu L,Chen Z,Zhao F,Zhang R,Lu A,Zhang G,Qian A.Targeting long noncoding RNA PMIF facilitates osteoprogenitor cells migrating to bone formation surface to promote bone formation during aging.Theranostics.2021 Mar 20;11(11):5585-5604.doi:10.7150/thno.54477. PMID:33859765;PMCID:PMC8039942.
Species: Human,Mouse
PA1103 usage in article: APP:WB, SAMPLE:HFOB1.19 CELL AND FEMUR TISSUE, DILUTION:1:1000

714: Concentration-dependent Effects of Nuclear Lamins on Nuclear Size in Xenopus and Mammalian Cells

Yang Lm, Liu Xl, Zhu Qt, Zhang Y, Xi Tf, Hu J, He Cf, Jiang L. J Biomed Mater Res B Appl Biomater. 2011 Jan;96(1):25-33. Doi: 10.1002/Jbm.B.31719. Human Peripheral Nerve-Derived Scaffold For Tissue-Engineered Nerve Grafts: Histology And Biocompati...

Liu J, Ma J, Wu Z, Li W, Zhang D, Han L, Wang F, Reindl Km, Wu E, Ma Q. Bmc Cancer. 2014 Sep 20;14:686. Doi: 10.1186/1471-2407-14-686. Arginine Deiminase Augments The Chemosensitivity Of Argininosuccinate Synthetase-Deficient Pancreatic Cancer Cel...

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16 Customer Q&As for Anti-Lamin A + C/LMNA Antibody

Question

Is a blocking peptide available for product anti-Lamin A + C/LMNA antibody (PA1103)?

Verified Customer

Verified customer

Asked: 2020-01-22

Answer

We do provide the blocking peptide for product anti-Lamin A + C/LMNA antibody (PA1103). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-01-22

Question

We want to test anti-Lamin A + C/LMNA antibody PA1103 on rat mucosa of stomach for research purposes, then I may be interested in using anti-Lamin A + C/LMNA antibody PA1103 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-12-20

Answer

The products we sell, including anti-Lamin A + C/LMNA antibody PA1103, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-12-20

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for mucosa of stomach using anti-Lamin A + C/LMNA antibody PA1103. Let me know if you need anything else.

P. Evans

Verified customer

Asked: 2019-12-03

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-03

Question

Can you help my question with product PA1103, anti-Lamin A + C/LMNA antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-11-18

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1103 anti-Lamin A + C/LMNA antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-11-18

Question

We have seen staining in human colon. Are there any suggestions? Is anti-Lamin A + C/LMNA antibody supposed to stain colon positively?

Verified Customer

Verified customer

Asked: 2019-11-14

Answer

According to literature colon does express LMNA. According to Uniprot.org, LMNA is expressed in mucosa of stomach, corpus callosum, kidney, lung skin, ovarian carcinoma, colon, renal cell carcinoma, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have LMNA expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 16964243, 17081983, 17924679, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon, Pubmed ID: 8621584
Corpus callosum, Pubmed ID: 14702039
Kidney, Lung, and Skin, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Renal cell carcinoma, Pubmed ID: 10508479, 10514485

Boster Scientific Support

Answered: 2019-11-14

Question

I see that the anti-Lamin A + C/LMNA antibody PA1103 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-10-31

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-10-31

Question

Is there a BSA free version of anti-Lamin A + C/LMNA antibody PA1103 available?

Verified Customer

Verified customer

Asked: 2019-09-27

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Lamin A + C/LMNA antibody PA1103 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-09-27

Question

We are currently using anti-Lamin A + C/LMNA antibody PA1103 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2019-07-11

Answer

The anti-Lamin A + C/LMNA antibody (PA1103) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-07-11

Question

Will PA1103 anti-Lamin A + C/LMNA antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

T. Parker

Verified customer

Asked: 2019-05-01

Answer

It shows on the product datasheet, PA1103 anti-Lamin A + C/LMNA antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-05-01

Question

I was wanting to use your anti-Lamin A + C/LMNA antibody for WB for rat mucosa of stomach on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat mucosa of stomach identification?

J. Jones

Verified customer

Asked: 2019-04-17

Answer

You can see on the product datasheet, PA1103 anti-Lamin A + C/LMNA antibody has been validated for IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat mucosa of stomach in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-04-17

Question

Will anti-Lamin A + C/LMNA antibody PA1103 work for WB with mucosa of stomach?

Verified Customer

Verified customer

Asked: 2018-11-21

Answer

According to the expression profile of mucosa of stomach, LMNA is highly expressed in mucosa of stomach. So, it is likely that anti-Lamin A + C/LMNA antibody PA1103 will work for WB with mucosa of stomach.

Boster Scientific Support

Answered: 2018-11-21

Question

We were happy with the WB result of your anti-Lamin A + C/LMNA antibody. However we have seen positive staining in cervix carcinoma nucleus. using this antibody. Is that expected? Could you tell me where is LMNA supposed to be expressed?

S. Mangal

Verified customer

Asked: 2018-03-05

Answer

Based on literature, cervix carcinoma does express LMNA. Generally LMNA expresses in nucleus., isoform c: nucleus speckle. Regarding which tissues have LMNA expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 16964243, 17081983, 17924679, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon, Pubmed ID: 8621584
Corpus callosum, Pubmed ID: 14702039
Kidney, Lung, and Skin, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Renal cell carcinoma, Pubmed ID: 10508479, 10514485

Boster Scientific Support

Answered: 2018-03-05

Question

Is this PA1103 anti-Lamin A + C/LMNA antibody reactive to the isotypes of LMNA?

Verified Customer

Verified customer

Asked: 2017-12-29

Answer

The immunogen of PA1103 anti-Lamin A + C/LMNA antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human Lamin A/C(455-469aa RNKSNEDQSMGNWQI), identical to the related rat and mouse sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-12-29

Question

Please see the WB image, lot number and protocol we used for mucosa of stomach using anti-Lamin A + C/LMNA antibody PA1103. Please let me know if you require anything else.

G. Li

Verified customer

Asked: 2017-04-04

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-04-04

Question

Would anti-Lamin A + C/LMNA antibody PA1103 work on primate ICC with corpus callosum?

R. Li

Verified customer

Asked: 2017-02-22

Answer

Our lab technicians have not validated anti-Lamin A + C/LMNA antibody PA1103 on primate. You can run a BLAST between primate and the immunogen sequence of anti-Lamin A + C/LMNA antibody PA1103 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate corpus callosum in ICC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-02-22

Question

We bought anti-Lamin A + C/LMNA antibody for WB on liver in a previous experiment. I am using rat, and We are going to use the antibody for IHC next. We need examining liver as well as cervix carcinoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?

C. Anderson

Verified customer

Asked: 2014-06-16

Answer

I looked at the website and datasheets of our anti-Lamin A + C/LMNA antibody and it seems that PA1103 has been validated on rat in both WB and IHC. Thus PA1103 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2014-06-16

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PA1103
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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