Anti-Lamin A + C/LMNA Antibody

Product Name

Anti-Lamin A + C/LMNA Antibody

View all LMNA Antibodies

SKU/Catalog Number

PA1103

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Lamin A + C/LMNA Antibody catalog # PA1103. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Lamin A + C/LMNA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1103)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Lamin A/C, identical to the related rat and mouse sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1103 is reactive to LMNA in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

70-74 kDa

Calculated molecular weight

74139 MW

Background of LMNA

Lamins are structural protein components of the nuclear lamina, a protein network underlying the inner nuclear membrane that determines nuclear shape and size. There are three types of lamins, A,B and C. The lamin A/C (LMNA) gene contains 12 exons. Alternative splicing within exon 10 gives rise to two different mRNAs that code for pre-lamin A and lamin C. Lamin A/C mapped to 1q21.2-q21.3 and mutations in this gene cause a variety of human diseases including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy, and Hutchinson-Gilford progeria syndrome. Lamin A/C deficiency is thus associated with both defective nuclear mechanics and impaired mechanically activated gene transcription.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA1103 is guaranteed for IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunocytochemistry , 0.5-1μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Positive Control

Validation Images & Assay Conditions

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Anti-Lamin A+C antibody, PA1103, IHC(P)
IHC(P): Rat Intestine Tissue

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Figure 1. Western blot analysis of Lamin A/C using anti-Lamin A/C antibody (PA1103).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: human CACO-2 whole cell lysates,
Lane 6: rat spleen tissue lysates,
Lane 7: mouse liver tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lamin A/C antigen affinity purified polyclonal antibody (Catalog # PA1103) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lamin A/C at approximately 70-74KD. The expected band size for Lamin A/C is at 74KD.

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Anti-Lamin A+C antibody, PA1103, IHC(P)
IHC(P): Human Lung Cancer Tissue

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Anti-Lamin A+C antibody, PA1103, IHC(P)
IHC(P): Mouse Intestine Tissue

Gene/Protein Information For LMNA (Source: Uniprot.org, NCBI)

Alternative Names

CDCD1; CDDC; CMD1A; CMT2B1; dilated 1A (autosomal dominant); EMD2; FPLD; HGPSFPL; lamin A/C; lamin A/C-like 1; lamin-A/C; LDP1; LFP; LGMD1B; limb girdle muscular dystrophy 1B (autosomal dominant); LMN1IDC; LMNC; LMNL1; prelamin-A/C; PRO1,70 kDa lamin; progeria 1 (Hutchinson-Gilford type); renal carcinoma antigen NY-REN-32 LMNA CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, FPLD2, HGPS, IDC, LDP1, LFP, LGMD1B, LMN1, LMNC, LMNL1, MADA, PRO1 lamin A/C lamin|70 kDa lamin|epididymis secretory sperm binding protein|lamin A/C-like 1|mandibuloacral dysplasia type A|prelamin-A/C|renal carcinoma antigen NY-REN-32

For more info on LMNA, check out the LMNA Infographic

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In this infographic, you will see the following information for LMNA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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