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Product Info Summary
| SKU: | M03522 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rat |
| Application: | Flow Cytometry, IF, IHC |
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Product info
Product Name
Anti-Laminin, gamma 1 (LAMC1) Monoclonal Antibody
View all Laminin gamma 1 Antibodies
SKU/Catalog Number
M03522
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-Laminin, gamma 1 (LAMC1) Monoclonal Antibody (Catalog # M03522). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Mouse.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-Laminin, gamma 1 (LAMC1) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03522)
Host
Rat
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: SPM193
Isotype
IgG2a, kappa
Immunogen
Murine EHS laminin preparation
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03522 is reactive to LAMC1 in Human, Mouse
Applications
M03522 is guaranteed for Flow Cytometry, IF, IHC Boster Guarantee
Observed Molecular Weight
19 kDa
Calculated molecular weight
177.603kDa
Background of Laminin gamma 1
0
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
Click image to see more details
Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Anti-Laminin Monoclonal Antibody (SPM193).
Protein Target Info & Infographic
Gene/Protein Information For LAMC1 (Source: Uniprot.org, NCBI)
Gene Name
LAMC1
Full Name
Laminin subunit gamma-1
Weight
177.603kDa
Alternative Names
LAMB2Laminin-7 subunit gamma; LAMC1; Laminin B2 chain; Laminin B2; Laminin gamma 1; laminin subunit gamma-1; laminin, gamma 1 (formerly LAMB2); Laminin-1 subunit gamma; Laminin-10 subunit gamma; Laminin-11 subunit gamma; Laminin-2 subunit gamma; Laminin-3 subunit gamma; Laminin-4 subunit gamma; Laminin-6 subunit gamma; Laminin-8 subunit gamma; Laminin-9 subunit gamma; MGC87297; S-LAM gamma; S-laminin subunit gamma LAMC1 LAMB2 laminin subunit gamma 1 laminin subunit gamma-1|S-LAM gamma|S-laminin subunit gamma|laminin B2 chain|laminin, gamma 1 (formerly LAMB2)|laminin-10 subunit gamma|laminin-11 subunit gamma|laminin-2 subunit gamma|laminin-3 subunit gamma|laminin-4 subunit gamma|laminin-6 subunit gamma|laminin-7 subunit gamma|laminin-8 subunit gamma|laminin-9 subunit gamma
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LAMC1, check out the LAMC1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LAMC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Laminin, gamma 1 (LAMC1) Monoclonal Antibody (M03522)
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No publications found for M03522
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Laminin, gamma 1 (LAMC1) Monoclonal Antibody
Question
Is this M03522 anti-Laminin, gamma 1 (LAMC1) Monoclonal antibody reactive to the isotypes of LAMC1?
Verified Customer
Verified customer
Asked: 2020-04-07
Answer
The immunogen of M03522 anti-Laminin, gamma 1 (LAMC1) Monoclonal antibody is Murine EHS laminin preparation. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-07
Question
Does anti-Laminin, gamma 1 (LAMC1) Monoclonal antibody M03522 work for IHC with liver?
Verified Customer
Verified customer
Asked: 2020-01-16
Answer
According to the expression profile of liver, LAMC1 is highly expressed in liver. So, it is likely that anti-Laminin, gamma 1 (LAMC1) Monoclonal antibody M03522 will work for IHC with liver.
Boster Scientific Support
Answered: 2020-01-16
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Laminin, gamma 1 (LAMC1) Monoclonal antibody M03522. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-04
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-04
Question
Please see the WB image, lot number and protocol we used for liver using anti-Laminin, gamma 1 (LAMC1) Monoclonal antibody M03522. Please let me know if you require anything else.
O. Anderson
Verified customer
Asked: 2018-11-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-13
Question
My question regarding product M03522, anti-Laminin, gamma 1 (LAMC1) Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-10-02
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M03522 anti-Laminin, gamma 1 (LAMC1) Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-10-02
Question
Would anti-Laminin, gamma 1 (LAMC1) Monoclonal antibody M03522 work on horse IHC with plasma?
Z. Anderson
Verified customer
Asked: 2016-06-23
Answer
Our lab technicians have not tested anti-Laminin, gamma 1 (LAMC1) Monoclonal antibody M03522 on horse. You can run a BLAST between horse and the immunogen sequence of anti-Laminin, gamma 1 (LAMC1) Monoclonal antibody M03522 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse plasma in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-06-23
Question
Is there a BSA free version of anti-Laminin, gamma 1 (LAMC1) Monoclonal antibody M03522 available?
L. Collins
Verified customer
Asked: 2013-01-22
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Laminin, gamma 1 (LAMC1) Monoclonal antibody M03522 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-01-22
