Anti-LIM domain-binding protein 2 LDB2 Antibody

Product Name

Anti-LIM domain-binding protein 2 LDB2 Antibody

View all LDB2 Antibodies

SKU/Catalog Number

A09871

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-LIM domain-binding protein 2 LDB2 Antibody (Catalog # A09871). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-LIM domain-binding protein 2 LDB2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09871)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 100-125 of Mouse LDB2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A09871 is reactive to LDB2 in Human, Mouse

Applications

A09871 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

42.793kDa

Background of LDB2

LDB2 (LIM homeobox protein cofactor CLIM-1a). It is suggested that LIM domain binding proteins (LDB1, LDB2 and LDB3) act synergistically to enhance transcriptional efficiency by acting as co-factors for LIM homeodomain and Otx class transcription factors both of which have essential roles in development. LDB2 is a close homologue of LDB1. LDBs are homologs of the Drosophila Chip protein. Three members of the ldb gene family (Ldb1, Ldb2 and Ldb3) from the zebrafish, Danio rerio, share 95%, 73% and 62% amino acid identity with mouse Ldb1, respectively.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:5,000 - 1:20,000
IHC: 5 µg/ml
WB: 1:500 - 1:2,000

Validation Images & Assay Conditions

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Western blot analysis of LDB2 expression in human kidney extract (lane 1) and mouse spleen extract (lane 2). LDB2 at 43KD was detected using rabbit anti-LDB2 Antigen Affinity purified polyclonal antibody (Catalog # A09871) at 1:500. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).

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LDB2 was detected in paraffin-embedded sections of human brain cortex tissues using rabbit anti-LDB2 Antigen Affinity purified polyclonal antibody (Catalog # A09871) at 5 μg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).

Gene/Protein Information For LDB2 (Source: Uniprot.org, NCBI)

Alternative Names

Carboxyl-terminal LIM domain-binding protein 1; CLIM-1; CLIM1LDB1; LDB-2; LIM binding domain 2; LIM domain binding 2; LIM domain-binding factor CLIM1; LIM domain-binding factor-2; LIM domain-binding protein 2 LDB2 CLIM1, LDB-2, LDB1 LIM domain binding 2 LIM domain-binding protein 2|LIM binding domain 2|LIM domain-binding factor CLIM1|LIM domain-binding factor-2|carboxyl-terminal LIM domain-binding protein 1

For more info on LDB2, check out the LDB2 Infographic

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In this infographic, you will see the following information for LDB2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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