Anti-LEF1 Rabbit Monoclonal Antibody

Product Name

Anti-LEF1 Rabbit Monoclonal Antibody

View all Lef1 Antibodies

SKU/Catalog Number

M00605

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-LEF1 Rabbit Monoclonal Antibody catalog # M00605. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-LEF1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00605)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

CFG-12

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human LEF1

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00605 is reactive to Lef1 in Human, Mouse, Rat

Applications

M00605 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

32-42 kDa, 65 kDa, 75 kDa

Calculated molecular weight

44.059kDa

Background of Lef1

Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:100-1:500
IP 1:50
FC 1:50

Validation Images & Assay Conditions

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Western blot analysis of LEF1 expression in Jurkat cell lysate.

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Immunohistochemical analysis of paraffin-embedded human spleen, using LEF1 Antibody.

Gene/Protein Information For Lef1 (Source: Uniprot.org, NCBI)

Alternative Names

DKFZp586H0919; FLJ46390; LEF1; LEF-1; lymphoid enhancer-binding factor 1; T cell-specific transcription factor 1-alpha; TCF10; TCF1ALPHA; TCF1-alpha; TCF7L3 Lef1|3000002B05, AI451430, Lef-, Lef-1|lymphoid enhancer binding factor 1|lymphoid enhancer-binding factor 1

For more info on Lef1, check out the Lef1 Infographic

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In this infographic, you will see the following information for Lef1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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