Product Info Summary
SKU: | A00350-4 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IHC, ICC, WB |
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Product info
Product Name
Anti-Leptin Receptor LEPR Antibody
SKU/Catalog Number
A00350-4
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Leptin Receptor LEPR Antibody catalog # A00350-4. Tested in WB,ICC,IHC,Flow Cytometry applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Leptin Receptor LEPR Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00350-4)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human RAD50.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00350-4 is reactive to LEPR in Human, Mouse, Rat
Applications
A00350-4 is guaranteed for Flow Cytometry, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
132.494kDa
Background of Leptin R
Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.
Dolganov G.M., Mol. Cell. Biol. 16:4832-4841(1996).
Kim K.K., Gene 235:59-67(1999).
Carney J.P., Cell 93:477-486(1998).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
FC: 1:50-1:100
WB: 1:500-1:1,000
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Immunocytochemistry staining of LEPR using Anti-Leptin Receptor LEPR Antibody (A00350-4).
ICC staining Leptin Receptor in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehydeClick image to see more details
Figure 2. Immunohistochemistry validation of LEPR using Anti-Leptin Receptor LEPR Antibody (A00350-4).
Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-Leptin Receptor antibody. Counter stained with hematoxylin.For more protocol information of IHC
Protein Target Info & Infographic
Gene/Protein Information For LEPR (Source: Uniprot.org, NCBI)
Gene Name
LEPR
Full Name
Leptin receptor
Weight
132.494kDa
Superfamily
type I cytokine receptor family
Alternative Names
B219; CD295 antigen; CD295; DB; DKFZp686B1731; huB219; LEPR; LEP-R; Leptin R; leptin receptor; LeptinR; OB R; OB receptor; OB-R; OBRCD295 LEPR CD295, LEP-RD, OB-R, OBR, LEPR leptin receptor leptin receptor|OB receptor|huB219
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LEPR, check out the LEPR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LEPR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Leptin Receptor LEPR Antibody (A00350-4)
Hello CJ!
A00350-4 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Srinivasan G,Parida S,Pavithra S, Panigrahi M,Sahoo M,Singh TU,Madhu CL,Manickam K,Shyamkumar TS,Kumar D,Mishra SK.Leptin receptor stimulation in late pregnant mouse uterine tissue inhibits spontaneous contractions by increasing NO and cGMP.Cytokine.2021
Species: Mouse
A00350-4 usage in article: APP:WB, SAMPLE:UTERINE TISSUE, DILUTION:1:300
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Customer Reviews
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