Product Info Summary
SKU: | PA1783 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-liver Arginase/ARG1 Antibody
View all Arginase 1/ARG1/liver Arginase Antibodies
SKU/Catalog Number
PA1783
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-liver Arginase/ARG1 Antibody catalog # PA1783. Tested in WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-liver Arginase/ARG1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1783)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of rat liver Arginase, different from the related mouse sequence by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1783 is reactive to ARG1 in Mouse, Rat
Applications
PA1783 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
38 kDa
Calculated molecular weight
34.735kDa
Background of Arginase 1/ARG1/liver Arginase
ARG1 (arginase, live) is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. The isoform encoded by ARG1, referred to as the liver, or A-I, isoform, contributes 98% of the arginase activity in liver but is also present in red cells. Using a rat liver ARG1 cDNA clone to probe a human liver cDNA library, Haraguchi et al. (1987) isolated and characterized a cDNA corresponding to the ARG1 gene. The ARG1 gene is mapped on 6q23.2 and the arginase gene contains 8 exons. By immunologic studies, 90% of the arginase in red blood cell and liver was precipitated by the antibody, whereas only 50% of the arginase in kidney, brain, and the gastrointestinal tract reacted with it. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of ARG1 using anti-ARG1 antibody (PA1783).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARG1 antigen affinity purified polyclonal antibody (Catalog # PA1783) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARG1 at approximately 38 kDa. The expected band size for ARG1 is at 38 kDa.
Protein Target Info & Infographic
Gene/Protein Information For ARG1 (Source: Uniprot.org, NCBI)
Gene Name
ARG1
Full Name
Arginase-1
Weight
34.735kDa
Superfamily
arginase family
Alternative Names
AI; ARG1; Arginase 1; arginase, liver; Arginase-1; EC 3.5.3.1; Liver Arginase; Liver-type arginase; PGIF; Type I Arginase ARG1 arginase 1 arginase-1|arginase, liver|liver-type arginase|type I arginase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ARG1, check out the ARG1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ARG1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-liver Arginase/ARG1 Antibody (PA1783)
Hello CJ!
PA1783 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Liao H,Li Y,Zhang X,Zhao X,Zheng D,Shen D,Li R. Protective Effects of Thalidomide on High-Glucose-Induced Podocyte Injury through In Vitro Modulation of Macrophage M1/M2 Differentiation.J Immunol Res.2020 Aug 27;2020:8263598.doi:10.1155/2020/ 8263598.PMID
Species: Mouse
PA1783 usage in article: APP:WB, SAMPLE:MACROPHAGES, DILUTION:1:500
CFTR protects against vascular inflammation and atherogenesis in apolipoprotein E-deficient mice
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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16 Customer Q&As for Anti-liver Arginase/ARG1 Antibody
Question
Is there a BSA free version of anti-liver Arginase/ARG1 antibody PA1783 available?
Verified Customer
Verified customer
Asked: 2020-03-04
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-liver Arginase/ARG1 antibody PA1783 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-04
Question
We have observed staining in mouse blood. What should we do? Is anti-liver Arginase/ARG1 antibody supposed to stain blood positively?
Verified Customer
Verified customer
Asked: 2020-03-02
Answer
Based on literature blood does express ARG1. Based on Uniprot.org, ARG1 is expressed in liver, blood, erythroblast, liver skeletal muscle, among other tissues. Regarding which tissues have ARG1 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 3174433
Erythroblast, Pubmed ID: 14574404
Liver, Pubmed ID: 2241902, 3540966, 24275569
Liver, and Skeletal muscle, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-03-02
Question
Will PA1783 anti-liver Arginase/ARG1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-12
Answer
It shows on the product datasheet, PA1783 anti-liver Arginase/ARG1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-12
Question
See attached the WB image, lot number and protocol we used for blood using anti-liver Arginase/ARG1 antibody PA1783. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-18
Question
My question regarding product PA1783, anti-liver Arginase/ARG1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-22
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1783 anti-liver Arginase/ARG1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-22
Question
I would like using your anti-liver Arginase/ARG1 antibody for cellular response to transforming growth factor beta stimulus studies. Has this antibody been tested with western blotting on tissue lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-08-16
Answer
We appreciate your inquiry. This PA1783 anti-liver Arginase/ARG1 antibody is validated on rat liver tissue, tissue lysate, kidney tissue. It is guaranteed to work for WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-08-16
Question
I was wanting to use your anti-liver Arginase/ARG1 antibody for WB for rat blood on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat blood identification?
T. Singh
Verified customer
Asked: 2019-05-24
Answer
You can see on the product datasheet, PA1783 anti-liver Arginase/ARG1 antibody has been tested for WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat blood in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-24
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using anti-liver Arginase/ARG1 antibody PA1783. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-04-25
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-04-25
Question
I am interested in to test anti-liver Arginase/ARG1 antibody PA1783 on rat blood for research purposes, then I may be interested in using anti-liver Arginase/ARG1 antibody PA1783 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-10-03
Answer
The products we sell, including anti-liver Arginase/ARG1 antibody PA1783, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-10-03
Question
Does anti-liver Arginase/ARG1 antibody PA1783 work on dog WB with liver?
Verified Customer
Verified customer
Asked: 2018-05-28
Answer
Our lab technicians have not validated anti-liver Arginase/ARG1 antibody PA1783 on dog. You can run a BLAST between dog and the immunogen sequence of anti-liver Arginase/ARG1 antibody PA1783 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-05-28
Question
Our lab were satisfied with the WB result of your anti-liver Arginase/ARG1 antibody. However we have observed positive staining in blood cytoplasm. using this antibody. Is that expected? Could you tell me where is ARG1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-05-18
Answer
According to literature, blood does express ARG1. Generally ARG1 expresses in cytoplasm. Regarding which tissues have ARG1 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 3174433
Erythroblast, Pubmed ID: 14574404
Liver, Pubmed ID: 2241902, 3540966, 24275569
Liver, and Skeletal muscle, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-05-18
Question
Is this PA1783 anti-liver Arginase/ARG1 antibody reactive to the isotypes of ARG1?
Z. Singh
Verified customer
Asked: 2017-06-16
Answer
The immunogen of PA1783 anti-liver Arginase/ARG1 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of rat liver Arginase(307-323aa KREGNHKPETDYLKPPK), different from the related mouse sequence by two amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-06-16
Question
We are currently using anti-liver Arginase/ARG1 antibody PA1783 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it true that the antibody can work on primate tissues as well?
N. Wu
Verified customer
Asked: 2015-08-06
Answer
The anti-liver Arginase/ARG1 antibody (PA1783) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-08-06
Question
Will anti-liver Arginase/ARG1 antibody PA1783 work for WB with blood?
L. Banerjee
Verified customer
Asked: 2015-01-15
Answer
According to the expression profile of blood, ARG1 is highly expressed in blood. So, it is likely that anti-liver Arginase/ARG1 antibody PA1783 will work for WB with blood.
Boster Scientific Support
Answered: 2015-01-15
Question
I see that the anti-liver Arginase/ARG1 antibody PA1783 works with WB, what is the protocol used to produce the result images on the product page?
T. Mangal
Verified customer
Asked: 2014-07-22
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2014-07-22
Question
Is a blocking peptide available for product anti-liver Arginase/ARG1 antibody (PA1783)?
A. Zhao
Verified customer
Asked: 2013-11-19
Answer
We do provide the blocking peptide for product anti-liver Arginase/ARG1 antibody (PA1783). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-11-19