Anti-Lunatic Fringe LFNG Antibody

Lunatic Fringe antibody

Boster Bio Anti-Lunatic Fringe LFNG Antibody catalog # A04513-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A04513-1
Size: 100μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, WB

Product Name

Anti-Lunatic Fringe LFNG Antibody

View all Lunatic Fringe Antibodies

SKU/Catalog Number

A04513-1

Size

100μl

Form

Liquid

Description

Boster Bio Anti-Lunatic Fringe LFNG Antibody catalog # A04513-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Lunatic Fringe LFNG Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04513-1)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

3F8

Isotype

IgG

Immunogen

Synthesized peptide derived from the Internal region of human Lunatic Fringe. at AA rangle: 90-170

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A04513-1 is reactive to LFNG in Human, Mouse, Rat

Applications

A04513-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

41.773kDa

Background of Lunatic Fringe

May play an important role in modulating bone turnover. Promotes the adhesion of osteoblast cells and inhibits the binding of fibrinogen to integrin receptors. In addition, inhibits osteocalcin production.

Pennica D., Swanson T.A., Welsh J.W. J.Proc. Natl. Acad. Sci. U.S.A. 95:14717-14722(1998)

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000
ELISA 1:10000

Validation Images & Assay Conditions

Gene/Protein Information For LFNG (Source: Uniprot.org, NCBI)

Gene Name

LFNG

Full Name

Beta-1,3-N-acetylglucosaminyltransferase lunatic fringe

Weight

41.773kDa

Superfamily

glycosyltransferase 31 family

Alternative Names

beta-1,3-N-acetylglucosaminyltransferase lunatic fringe; EC 2.4.1.222; LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase; LFNG; lunatic fringe (Drosophila) homolog; lunatic fringe homolog (Drosophila); Lunatic Fringe; O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase; SCDO3; SCDO3EC 2.4.1.222 LFNG SCDO3 LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase beta-1,3-N-acetylglucosaminyltransferase lunatic fringe

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on LFNG, check out the LFNG Infographic

LFNG infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for LFNG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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13 Customer Q&As for Anti-Lunatic Fringe LFNG Antibody

Question

Is this A04513-1 anti-Lunatic Fringe antibody reactive to the isotypes of LFNG?

Verified Customer

Verified customer

Asked: 2020-01-13

Answer

The immunogen of A04513-1 anti-Lunatic Fringe antibody is Synthesized peptide derived from the Internal region of human Lunatic Fringe. at AA rangle: 90-170. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-01-13

Question

See below the WB image, lot number and protocol we used for vagina using anti-Lunatic Fringe antibody A04513-1. Please let me know if you require anything else.

K. Mitchell

Verified customer

Asked: 2019-12-24

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-24

Question

My question regards to test anti-Lunatic Fringe antibody A04513-1 on rat vagina for research purposes, then I may be interested in using anti-Lunatic Fringe antibody A04513-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-11-12

Answer

The products we sell, including anti-Lunatic Fringe antibody A04513-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-11-12

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for vagina using anti-Lunatic Fringe antibody A04513-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-06-25

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-06-25

Question

Does A04513-1 anti-Lunatic Fringe antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

E. Patel

Verified customer

Asked: 2018-11-29

Answer

It shows on the product datasheet, A04513-1 anti-Lunatic Fringe antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-11-29

Question

I see that the anti-Lunatic Fringe antibody A04513-1 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-11-06

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-11-06

Question

Will anti-Lunatic Fringe antibody A04513-1 work on goat WB with vagina?

Verified Customer

Verified customer

Asked: 2018-04-30

Answer

Our lab technicians have not tested anti-Lunatic Fringe antibody A04513-1 on goat. You can run a BLAST between goat and the immunogen sequence of anti-Lunatic Fringe antibody A04513-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat vagina in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-04-30

Question

I was wanting to use your anti-Lunatic Fringe antibody for WB for rat vagina on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat vagina identification?

Verified Customer

Verified customer

Asked: 2017-12-14

Answer

It shows on the product datasheet, A04513-1 anti-Lunatic Fringe antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat vagina in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-12-14

Question

Is a blocking peptide available for product anti-Lunatic Fringe antibody (A04513-1)?

V. Anderson

Verified customer

Asked: 2017-09-18

Answer

We do provide the blocking peptide for product anti-Lunatic Fringe antibody (A04513-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-09-18

Question

Do you have a BSA free version of anti-Lunatic Fringe antibody A04513-1 available?

Verified Customer

Verified customer

Asked: 2017-09-15

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Lunatic Fringe antibody A04513-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-09-15

Question

Can you help my question with product A04513-1, anti-Lunatic Fringe antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2017-09-05

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04513-1 anti-Lunatic Fringe antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-09-05

Question

We are currently using anti-Lunatic Fringe antibody A04513-1 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?

C. Taylor

Verified customer

Asked: 2014-09-04

Answer

The anti-Lunatic Fringe antibody (A04513-1) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2014-09-04

Question

Will anti-Lunatic Fringe antibody A04513-1 work for WB with vagina?

C. Bhatt

Verified customer

Asked: 2013-09-02

Answer

According to the expression profile of vagina, LFNG is highly expressed in vagina. So, it is likely that anti-Lunatic Fringe antibody A04513-1 will work for WB with vagina.

Boster Scientific Support

Answered: 2013-09-02

Order DetailsPrice
A04513-1

100uL

$399

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A04513-1
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$399.00

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