Click image to see more details
Product Info Summary
| SKU: | M00951 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-M6PR IGF2R Rabbit Monoclonal Antibody
SKU/Catalog Number
M00951
BM4355 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-M6PR IGF2R Rabbit Monoclonal Antibody catalog # M00951. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-M6PR IGF2R Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00951)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
DIC-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human M6PR
Reactive Species
M00951 is reactive to IGF2R in Human, Mouse, Rat
Observed Molecular Weight
300 kDa
Calculated molecular weight
274.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00951 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-5000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:30
FC 1:100
Positive Control
WB: human HepG2 whole cell, human THP-1 whole cell, human U251 whole cell, human SiHa whole cell, rat heart tissue, rat lung tissue, mouse heart tissue, mouse lung tissue
IHC: human colon tissue
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of IGF2R using anti-IGF2R antibody (M00951).
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human THP-1 whole cell lysates,
Lane 3: human U251 whole cell lysates,
Lane 4: human SiHa whole cell lysates,
Lane 5: rat heart tissue lysates,
Lane 6: rat lung tissue lysates,
Lane 7: mouse heart tissue lysates,
Lane 8: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGF2R antigen affinity purified monoclonal antibody (M00951) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IGF2R at approximately 300 kDa. The expected band size for IGF2R is at 274 kDa.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human colon, using M6PR/IGF2R Antibody.
Specific Publications For Anti-M6PR IGF2R Rabbit Monoclonal Antibody (M00951)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-M6PR IGF2R Rabbit Monoclonal Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-M6PR IGF2R Rabbit Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
