Anti-MACC1 Antibody

Product Name

Anti-MACC1 Antibody

View all MACC1 Antibodies

SKU/Catalog Number

A04732

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-MACC1 Antibody (Catalog # A04732). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

MACC1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-MACC1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04732)

Host

Rabbit

Contents

MACC1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

Clone: SK7

Isotype

IgG

Immunogen

MACC1 antibody was raised against a 17 amino acid synthetic peptide near the center of human MACC1. The immunogen is located within amino acids 430 - 480 of MACC1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least two isoforms of MACC1 are known to exist.

Reactive Species

A04732 is reactive to MACC1 in Human, Mouse, Rat

Applications

A04732 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

96.639kDa

Background of MACC1

Metastasis associated in colon cancer 1 (MACC1) is a key regulator of the hepatocyte growth factor (HGF)-HGF receptor (MET) pathway, which is involved in cellular growth, epithelial-mesenchymal transition, angiogenesis, cell motility, invasiveness, and metastasis. MACC1 protein consists of four domains: ZU5, SH3, and two C-terminal death domains (DD). Expression of MACC1 was found significantly upregulated in malignant tissues (colon cancer of all stages as well as liver and lung metastases) compared to normal tissues or adenomas. MACC1 represents an early and crucial prognostic indicator for colon cancer metastasis that is independent of age, sex, tumor infiltration, nodal status, and lymph vessel invasion. Besides its involvement in signal transduction with the MET receptor, MACC1 also links MET signaling and apoptosis. MACC1 may also be an important therapeutic target for colorectal cancer treatment.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

MACC1 antibody can be used for detection of MACC1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Click image to see more details

Western blot analysis of MACC1 in mouse liver tissue lysate with MACC1 antibody at (A) 1 and (B) 2 μg/mL.

Click image to see more details

Immunohistochemistry of MACC1 in human liver tissue with MACC1 antibody at 2.5 μg/mL.

Click image to see more details

Immunofluorescence of MACC1 in Human Liver cells with MACC1 antibody at 20 μg/mL.

Click image to see more details

Western blot analysis of MACC1 expression in human liver tissue lysate with MACC1 antibody at (A) 1 and (B) 2 μg/ml.

Gene/Protein Information For MACC1 (Source: Uniprot.org, NCBI)

Alternative Names

metastasis associated in colon cancer 1; SH3 domain-containing protein 7a5,7A5 MACC1 7A5, SH3BP4L MET transcriptional regulator MACC1 metastasis-associated in colon cancer protein 1|MACC1, MET transcriptional regulator|SH3 domain-containing protein 7a5|metastasis associated in colon cancer 1|putative binding protein 7a5

For more info on MACC1, check out the MACC1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MACC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the MACC1 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis