Anti-MADD Antibody

Product Name

Anti-MADD Antibody

View all MADD Antibodies

SKU/Catalog Number

A02030

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-MADD Antibody (Catalog # A02030). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MADD Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02030)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human MADD.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A02030 is reactive to MADD in Human, Mouse, Rat

Applications

A02030 is guaranteed for IF, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

183.303kDa

Background of MADD

Plays a significant role in regulating cell proliferation, survival and death through alternative mRNA splicing. Isoform 5 shows increased cell proliferation and isoform 2 shows decreased. Converts GDP-bound inactive form of RAB3A, RAB3C and RAB3D to the GTP-bound active forms. Component of the TNFRSF1A signaling complex: MADD links TNFRSF1A with MAP kinase activation. Plays an important regulatory role in physiological cell death (TNF-alpha-induced, caspase-mediated apoptosis); isoform 1 is susceptible to inducing apoptosis, isoform 5 is resistant and isoform 3 and isoform 4 have no effect.

Chow V.T.K., DNA Seq. 6:263-273(1996).
Schievella A.R., J. Biol. Chem. 272:12069-12075(1997).
Chow V.T.K., Genome 41:543-552(1998).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500

Validation Images & Assay Conditions

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Immunohistochemistry analysis of paraffin-embedded human brain tissue using MADD antibody A02030.

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Western blot analysis of extracts from COLO cells, HeLa cells and Jurkat cells, using MADD antibody A02030.

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Immunofluorescence analysis of A549 cells, using MADD antibody A02030.

Gene/Protein Information For MADD (Source: Uniprot.org, NCBI)

Alternative Names

MAP kinase-activating death domain protein MADD DEEAH, DENN, IG20, NEDDISH, RAB3GEP MAP kinase activating death domain MAP kinase-activating death domain protein|Rab3 GDP/GTP exchange factor|differentially expressed in normal and neoplastic cells|insulinoma glucagonoma clone 20

For more info on MADD, check out the MADD Infographic

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In this infographic, you will see the following information for MADD: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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