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Product Info Summary
| SKU: | A01524 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-MAP1LC3B Antibody
SKU/Catalog Number
A01524
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-MAP1LC3B Antibody (Catalog # A01524). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
MAP1LC3B antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-MAP1LC3B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01524)
Host
Rabbit
Contents
MAP1LC3B antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
MAP1LC3B antibody was raised against an 18 amino acid peptide near the amino terminus of human MAP1LC3B. The immunogen is located within the first 50 amino acids of MAP1LC3B.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
MAP1LC3B antibody is human, mouse and rat reactive. Multiple isoforms MAP1LC3B are known to exist. MAP1LC3B antibody is predicted to not cross-react with MAP1LC3A or MAP1LC3C
Reactive Species
A01524 is reactive to Map1lc3b in Human, Mouse, Rat
Applications
A01524 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
16.394kDa
Background of LC3B
Microtubule-associated proteins (MAPs) regulate microtubule stability and play critical roles in neuronal development and plasticity (1). MAP1LC3B belongs to the MAP1 LC3 family and it includes 3 different light chains, LC1, LC2 and LC3 (2). MAP1LC3B is involved in formation of autophagosomal vacuoles (autophagosomes) (3). It is most abundant in heart, brain, skeletal muscle and testis. MAP1LC3B is essential for autophagy and associated to the autophagosome membranes after processing (4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
MAP1LC3B antibody can be used for detection of MAP1LC3B by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of MAP1LC3B in human brain tissue lysate with MAP1LC3B antibody at 1 μg/ml.
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Immunohistochemistry of MAP1LC3B in human brain tissue with MAP1LC3B antibody at 5 μg/mL.
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Immunofluorescence of MAP1LC3B in human brain tissue with MAP1LC3B antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For Map1lc3b (Source: Uniprot.org, NCBI)
Gene Name
Map1lc3b
Full Name
Microtubule-associated proteins 1A/1B light chain 3B
Weight
16.394kDa
Superfamily
ATG8 family
Alternative Names
LC3B; Apg8b; atg8f; ATG8F; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; lc3b autophagy marker; LC3B ihc; LC3B immunohistochemistry; LC3B immunoprecipitation; LC3B western blot; LC3B; lc3-i, ii; LC3II; MAP1 light chain 3-like protein 2; MAP1A/1BLC3; MAP1A/MAP1B LC3 B; MAP1LC3B; MAP1LC3B-a; microtubule associated protein 1 light chain 3 beta; microtubule associated protein 3 b; microtubule associated protein 3 beta; microtubule-associated proteins 1A/1B light chain 3; microtubule-associated proteins 1A/1B light chain 3B Map1lc3b|LC3B, Map1lc3, Mpl3, zbs559|microtubule-associated protein 1 light chain 3 beta|microtubule-associated proteins 1A/1B light chain 3B|MAP1 light chain 3-like protein 2|MAP1A/1B light chain 3 B|MAP1A/MAP1B LC3 B|MAP1A/MAP1B light chain 3 B|autophagy-related protein LC3 B|autophagy-related ubiquitin-like modifier LC3 B|microtubule-associated proteins 1A/1B light chain 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Map1lc3b, check out the Map1lc3b Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Map1lc3b: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MAP1LC3B Antibody (A01524)
Hello CJ!
A01524 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhang Tao,Xiaoqing Zhou,Yan Zhang,Wenfeng Pu,Yi Yang,Fuxia Wei,Qian Zhou,Lin Zhang,Zhonghan Du,Ji Wu,"Xi Lei San Attenuates Dextran Sulfate Sodium-Induced Colitis in Rats and TNF-α-Stimulated Colitis in CACO2 Cells: Involvement of the NLRP3 Inflammasome and Autophagy",Mediators of Inflammation,vol. 2021, Article ID 1610251,12 pages,2021.https://doi.org/10.1155/2021/1610251
Species: Human,Rat
A01524 usage in article: APP:WB, SAMPLE:COLON TISSUE AND CACO2 CELL, DILUTION:1:800
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