Product Info Summary
SKU: | A05355-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-MARK3 Antibody
SKU/Catalog Number
A05355-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-MARK3 Antibody catalog # A05355-1. Tested in WB,IP,ICC/IF,IHC,Flow Cytometry applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MARK3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05355-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from N-terminal of human MARK3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05355-1 is reactive to MARK3 in Human, Mouse, Rat
Applications
A05355-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
111 kDa
Calculated molecular weight
84.429kDa
Background of MARK3
Involved in the specific phosphorylation of microtubule-associated proteins for tau, MAP2 and MAP4. Phosphorylates CDC25C on 'Ser-216'. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus.
Peng C.Y., Cell Growth Differ. 9:197-208(1998).
Sun T.-Q., Nat. Cell Biol. 3:628-636(2001).
Wissing J., Mol. Cell. Proteomics 6:537-547(2007)
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500
IP: 1:10-1:50
ICC: 1:50-1:200
IHC: 1:50-1:200
FC: 1:50-1:100
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunocytochemistry staining of MARK3 using Anti-MARK3 Antibody (A05355-1).
Immunocytochemistry staining MARK3 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with MARK3 monoclonal antibody at a dilution of 1:100 for 1 hour at room temperatureClick image to see more details
Figure 1. Western blotting validation for Anti-MARK3 Antibody A05355-1
Western blot analysis of MARK3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG -HRP Secondary Antibody (HA1001) at 1:5Protein Target Info & Infographic
Gene/Protein Information For MARK3 (Source: Uniprot.org, NCBI)
Gene Name
MARK3
Full Name
MAP/microtubule affinity-regulating kinase 3
Weight
84.429kDa
Superfamily
protein kinase superfamily
Alternative Names
Cdc25C-associated protein kinase 1; cTAK1; C-TAK1; CTAK1ELKL motif kinase 2; EC 2.7.11; EC 2.7.11.1; EMK2; EMK-2; KP78; MAP/microtubule affinity-regulating kinase 3; PAR1A; Protein kinase STK10; Ser/Thr protein kinase PAR-1; Serine/threonine-protein kinase p78 MARK3 CTAK1, KP78, PAR1A, Par-1a, VIPB microtubule affinity regulating kinase 3 MAP/microtubule affinity-regulating kinase 3|C-TAK1|ELKL motif kinase 2|EMK-2|cdc25C-associated protein kinase 1|protein kinase STK10|ser/Thr protein kinase PAR-1|serine/threonine-protein kinase p78
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MARK3, check out the MARK3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MARK3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MARK3 Antibody (A05355-1)
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No publications found for A05355-1
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Customer Reviews
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