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Product Info Summary
| SKU: | A06515-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-MCCC2 Antibody (Center)
SKU/Catalog Number
A06515-1
Size
80 µl
Description
Boster Bio Anti-MCCC2 Antibody (Center) (Catalog # A06515-1). Tested in WB, IHC-P, Flow Cytometry application(s). This antibody reacts with Human, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-MCCC2 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06515-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This MCCC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 163-189 amino acids from the Central region of human MCCC2.
Reactive Species
A06515-1 is reactive to MCCC2 in Human, Rat
Calculated molecular weight
61.3 kDa
Background of MCCC2
MCCC2 is the small subunit of 3-methylcrotonyl-CoA carboxylase. This enzyme functions as a heterodimer and catalyzes the carboxylation of 3-methylcrotonyl-CoA to form 3-methylglutaconyl-CoA.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06515-1 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:10-1:50
FC: 1:10-1:50
Validation Images & Assay Conditions
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MCCC2 Antibody (Center) western blot analysis in NCI-H292 cell line and rat liver tissue lysates (35ug/lane).This demonstrates the MCCC2 antibody detected the MCCC2 protein (arrow).
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Formalin-fixed and paraffin-embedded human hepatocarcinoma with MCCC2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Flow cytometric analysis of ATDC5 cells using MCCC2 Antibody (Center) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Specific Publications For Anti-MCCC2 Antibody (Center) (A06515-1)
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