Anti-MCM7 / CDC47 (Proliferation Marker) Monoclonal Antibody

Product Name

Anti-MCM7 / CDC47 (Proliferation Marker) Monoclonal Antibody

View all MCM7 Antibodies

SKU/Catalog Number

M01649

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-MCM7 / CDC47 (Proliferation Marker) Monoclonal Antibody (Catalog # M01649). Tested in Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-MCM7 / CDC47 (Proliferation Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01649)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: SPM379

Isotype

IgG1, kappa

Immunogen

Recombinant human hCDC47 protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M01649 is reactive to MCM7 in Human

Applications

M01649 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

81.308kDa

Background of MCM7

MCM7 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that is essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumor suppressor protein RB1/RB.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

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Formalin-fixed, paraffin-embedded human Tonsil stained with Anti-MCM7 Monoclonal Antibody (SPM379).

Gene/Protein Information For MCM7 (Source: Uniprot.org, NCBI)

Alternative Names

CDC47 homolog; CDC47; CDC47P85MCM; DNA replication licensing factor MCM7; EC 3.6.4.12; homolog of S. cerevisiae Cdc47; MCM2; MCM2PNAS146; MCM7 minichromosome maintenance deficient 7 (S. cerevisiae); MCM7; minichromosome maintenance complex component 7; minichromosome maintenance deficient (S. cerevisiae) 7; minichromosome maintenance deficient 7; P1.1-MCM3; P1CDC47; P85MCM; PNAS146; PPP1R104; Protein Phosphatase 1 MCM7 CDC47, MCM2, P1.1-MCM3, P1CDC47, P85MCM, PNAS146, PPP1R104 minichromosome maintenance complex component 7 DNA replication licensing factor MCM7|CDC47 homolog|homolog of S. cerevisiae Cdc47|minichromosome maintenance deficient 7|protein phosphatase 1, regulatory subunit 104

For more info on MCM7, check out the MCM7 Infographic

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In this infographic, you will see the following information for MCM7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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