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Product Info Summary
| SKU: | PB9562 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC |
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Product info
Product Name
Anti-MCSF/CSF1 Antibody
SKU/Catalog Number
PB9562
PB0588 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MCSF/CSF1 Antibody catalog # PB9562. Tested in ELISA, IHC applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MCSF/CSF1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9562)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse MCSF recombinant protein (Position: K33-E262). Mouse MCSF shares 80.9 % and 88.6% amino acid (aa) sequence identity with human and rat MCSF, respectively.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9562 is reactive to Csf1 in Mouse, Rat
Calculated molecular weight
60.6 kDa
Background of Csf1
M-CSF (or CSF-1) is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival ofmonocytes, macrophages, and bone marrow progenitor cells. M-CSF affects macrophages and monocytes in several ways, including stimulating increased phagocytic and chemotactic activity, and increased tumour cell cytotoxicity. The role of M-CSF is not only restricted to the monocyte/macrophage cell lineage. By interacting with its membrane receptor (CSF1R or M-CSF-R encoded by the c-fms proto-oncogene), M-CSF also modulates the proliferation of earlier hematopoietic progenitors and influence numerous physiological processes involved in immunology, metabolism, fertility and pregnancy.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9562 is guaranteed for ELISA, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA, 0.1-0.5μg/ml, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat
Positive Control
IHC: mouse spleen tissue, rat spleen tissue
Validation Images & Assay Conditions
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IHC analysis of MCSF using anti-MCSF antibody (PB9562).
MCSF was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-MCSF Antibody (PB9562) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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IHC analysis of MCSF using anti-MCSF antibody (PB9562).
MCSF was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-MCSF Antibody (PB9562) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Specific Publications For Anti-MCSF/CSF1 Antibody (PB9562)
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5 Customer Q&As for Anti-MCSF/CSF1 Antibody
Question
We have seen staining in mouse kidney. Are there any suggestions? Is anti-MCSF/CSF1 antibody supposed to stain kidney positively?
Verified Customer
Verified customer
Asked: 2019-07-18
Answer
From what I have seen in literature kidney does express CSF1. From what I have seen in Uniprot.org, CSF1 is expressed in esophagus, pancreatic carcinoma urine, trophoblast urine, pancreatic carcinoma, t lymphoblast, placenta, kidney, endometrium, erythroleukemia, among other tissues. Regarding which tissues have CSF1 expression, here are a few articles citing expression in various tissues:
Endometrium, Pubmed ID: 1791839
Erythroleukemia, Pubmed ID: 23186163
Kidney, Pubmed ID: 15489334
Pancreatic carcinoma, Pubmed ID: 2460758, 3500041
Pancreatic carcinoma, and Urine, Pubmed ID: 2996129
Placenta, Pubmed ID: 14702039
T lymphoblast, Pubmed ID: 2660794, 8262907
Trophoblast, and Urine, Pubmed ID: 3493529
Boster Scientific Support
Answered: 2019-07-18
Question
I am interested in using your anti-MCSF/CSF1 antibody for positive regulation of macrophage derived foam cell differentiation studies. Has this antibody been tested with western blotting on mouse spleen tissue? We would like to see some validation images before ordering.
V. Krishna
Verified customer
Asked: 2019-07-15
Answer
We appreciate your inquiry. This PB9562 anti-MCSF/CSF1 antibody is validated on mouse spleen tissue, rat spleen tissue. It is guaranteed to work for ELISA, IHC in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-07-15
Question
We are currently using anti-MCSF/CSF1 antibody PB9562 for rat tissue, and we are content with the ELISA results. The species of reactivity given in the datasheet says mouse, rat. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2018-06-22
Answer
The anti-MCSF/CSF1 antibody (PB9562) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-06-22
Question
Our lab used your anti-MCSF/CSF1 antibody for ELISA on pancreatic carcinoma urine in the past. I am using rat, and We are going to use the antibody for IHC next. I am interested in examining pancreatic carcinoma urine as well as trophoblast urine in our next experiment. Do you have any suggestion on which antibody would work the best for IHC?
H. Edwards
Verified customer
Asked: 2013-07-04
Answer
I looked at the website and datasheets of our anti-MCSF/CSF1 antibody and it seems that PB9562 has been tested on rat in both ELISA and IHC. Thus PB9562 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2013-07-04
Question
We were satisfied with the WB result of your anti-MCSF/CSF1 antibody. However we have observed positive staining in pancreatic carcinoma cell membrane using this antibody. Is that expected? Could you tell me where is CSF1 supposed to be expressed?
K. Roberts
Verified customer
Asked: 2013-04-19
Answer
According to literature, pancreatic carcinoma does express CSF1. Generally CSF1 expresses in cell membrane. Regarding which tissues have CSF1 expression, here are a few articles citing expression in various tissues:
Endometrium, Pubmed ID: 1791839
Erythroleukemia, Pubmed ID: 23186163
Kidney, Pubmed ID: 15489334
Pancreatic carcinoma, Pubmed ID: 2460758, 3500041
Pancreatic carcinoma, and Urine, Pubmed ID: 2996129
Placenta, Pubmed ID: 14702039
T lymphoblast, Pubmed ID: 2660794, 8262907
Trophoblast, and Urine, Pubmed ID: 3493529
Boster Scientific Support
Answered: 2013-04-19
