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Product Info Summary
| SKU: | M00620 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-MCSF Rabbit Monoclonal Antibody
SKU/Catalog Number
M00620
BM4237 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-MCSF Rabbit Monoclonal Antibody catalog # M00620. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MCSF Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00620)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
CGI-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human MCSF
Reactive Species
M00620 is reactive to CSF1 in Human
Observed Molecular Weight
20 kDa
Calculated molecular weight
60.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00620 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:1000
IHC 1:50-1:200
ICC/IF 1:50-1:100
IP 1:50
Validation Images & Assay Conditions
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Western blot analysis of MCSF expression in MCSF recombinant protein.
Specific Publications For Anti-MCSF Rabbit Monoclonal Antibody (M00620)
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4 Customer Q&As for Anti-MCSF Rabbit Monoclonal Antibody
Question
We ordered your anti-MCSF Rabbit Monoclonal antibody for WB on kidney last year. I am using human, and We are going to use the antibody for IF next. I am looking for examining kidney as well as t lymphoblast in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2019-10-30
Answer
I have checked the website and datasheets of our anti-MCSF Rabbit Monoclonal antibody and I see that M00620 has been tested on human in both WB and IF. Thus M00620 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-10-30
Question
We have observed staining in human pancreatic carcinoma urine. Any tips? Is anti-MCSF Rabbit Monoclonal antibody supposed to stain pancreatic carcinoma urine positively?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
From literature pancreatic carcinoma urine does express CSF1. From Uniprot.org, CSF1 is expressed in esophagus, pancreatic carcinoma urine, trophoblast urine, pancreatic carcinoma, t lymphoblast, placenta, kidney, endometrium, erythroleukemia, among other tissues. Regarding which tissues have CSF1 expression, here are a few articles citing expression in various tissues:
Endometrium, Pubmed ID: 1791839
Erythroleukemia, Pubmed ID: 23186163
Kidney, Pubmed ID: 15489334
Pancreatic carcinoma, Pubmed ID: 2460758, 3500041
Pancreatic carcinoma, and Urine, Pubmed ID: 2996129
Placenta, Pubmed ID: 14702039
T lymphoblast, Pubmed ID: 2660794, 8262907
Trophoblast, and Urine, Pubmed ID: 3493529
Boster Scientific Support
Answered: 2019-10-23
Question
We were content with the WB result of your anti-MCSF Rabbit Monoclonal antibody. However we have been able to see positive staining in erythroleukemia cell membrane using this antibody. Is that expected? Could you tell me where is CSF1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-10-04
Answer
From what I have seen in literature, erythroleukemia does express CSF1. Generally CSF1 expresses in cell membrane. Regarding which tissues have CSF1 expression, here are a few articles citing expression in various tissues:
Endometrium, Pubmed ID: 1791839
Erythroleukemia, Pubmed ID: 23186163
Kidney, Pubmed ID: 15489334
Pancreatic carcinoma, Pubmed ID: 2460758, 3500041
Pancreatic carcinoma, and Urine, Pubmed ID: 2996129
Placenta, Pubmed ID: 14702039
T lymphoblast, Pubmed ID: 2660794, 8262907
Trophoblast, and Urine, Pubmed ID: 3493529
Boster Scientific Support
Answered: 2019-10-04
Question
We are currently using anti-MCSF Rabbit Monoclonal antibody M00620 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-11
Answer
The anti-MCSF Rabbit Monoclonal antibody (M00620) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-11
