Anti-ME1 Antibody Picoband™ (monoclonal, 5E5F7)

Product Name

Anti-ME1 Antibody Picoband™ (monoclonal, 5E5F7)

View all ME1 Antibodies

SKU/Catalog Number

M03449

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-ME1 Antibody Picoband™ (monoclonal, 5E5F7) catalog # M03449. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-ME1 Antibody Picoband™ (monoclonal, 5E5F7) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03449)

Host

Mouse

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Monoclonal

Clone Number

5E5F7

Isotype

Mouse IgG1

Immunogen

E.coli-derived human ME1 recombinant protein (Position: M1-Q572).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

M03449 is reactive to ME1 in Human, Mouse, Rat

Applications

M03449 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

64 kDa

Calculated molecular weight

64.15kDa

Background of ME1

NADP-dependent malic enzyme is a protein that in humans is encoded by the ME1 gene. This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Rat

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of ME1 using anti-ME1 antibody (M03449).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: rat testis tissue lysates,
Lane 4: rat brain tissue lysates,
Lane 5: mouse testis tissue lysates,
Lane 6: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-ME1 antigen affinity purified monoclonal antibody (Catalog # M03449) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for ME1 at approximately 64 kDa. The expected band size for ME1 is at 64 kDa.

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Figure 2. IHC analysis of ME1 using anti-ME1 antibody (M03449).
ME1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-ME1 Antibody (M03449) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.

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Figure 3. IHC analysis of ME1 using anti-ME1 antibody (M03449).
ME1 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-ME1 Antibody (M03449) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.

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Figure 4. IHC analysis of ME1 using anti-ME1 antibody (M03449).
ME1 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-ME1 Antibody (M03449) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.

Gene/Protein Information For ME1 (Source: Uniprot.org, NCBI)

Alternative Names

EC 1.1.1; EC 1.1.1.40; HUMNDME; malate dehydrogenase; Malic enzyme 1; malic enzyme 1, NADP(+)-dependent, cytosolic; malic enzyme 1, soluble; Malic enzyme, cytoplasmic; MES; NADP-dependent malic enzyme; NADP-ME; pyruvic-malic carboxylase ME1 HUMNDME, MES malic enzyme 1 NADP-dependent malic enzyme|Malic enzyme, cytoplasmic|NADP-ME|malate dehydrogenase (oxaloacetate-decarboxylating) (NADP(+))|malic enzyme 1, NADP(+)-dependent, cytosolic|malic enzyme 1, soluble|pyruvic-malic carboxylase

For more info on ME1, check out the ME1 Infographic

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In this infographic, you will see the following information for ME1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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